Dataset Information

The role of DENND10 in neuronal development

ABSTRACT: Neuron development requires an elaborate network of endosomal transport pathways, defects in which underlie multiple neurological diseases. DENND10/FAM45A is an ancient protein that is important for endosomal homeostasis. However, the role of DENND10 in the nervous system remained unknown. Here, we impaired DENND10 expression in CAD neuronal cells by CRISPR editing. DENND10 deficiency resulted in shortened neurites, which was rescued by the re-expression of DENND10. Expression levels of neuronal specific cytoskeleton protein Tubb3 and tyrosine hydroxylase (Th) were also suppressed by DENND10 deletion. Here we utilized TMT-based quantitative mass spectrometry to study the proteomic landscape of DENND10-impaired CAD neurons.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Yanling Zhang

LAB HEAD: Yanling Zhang

PROVIDER: PXD038623 | Pride | 2025-03-06

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 14

Similar Datasets

Project description:Canine adipose-derived mesenchymal stem cells (cAD-MSCs) show therapeutic promise for their regenerative potential, particularly in their secretome. However, concerns arise regarding the impact of in vitro cultivation need for storing therapeutic doses, prompting this study to comprehensively explore the impact of in vitro aging on gene expression and secretome composition. The study involved collecting abdominal adipose tissue samples from nine healthy female dogs, from which cAD-MSCs were extracted and cultured. Stem cells were validated through trilineage differentiation assays and flow cytometry immunophenotyping. Gene expression profiling, using RT-qPCR array and cAD-MSCs secretome LC-MS/MS analysis, were conducted at passages 3 and 6 to reveal gene expression and protein composition alterations during in vitro culture. Gene expression profiling of in vitro aged cAD-MSCs revealed expression alterations, while significant downregulation was observed in two MSC-associated genes. Proteomic analysis revealed 10% distinctively expressed proteins, and several up- and downregulations. Grouping these proteins with biologically significant ones, Gene Ontology Panther Pathway analysis revealed that P3 proteins were significantly associated with cytoskeletal regulation, nicotinic acetylcholine receptor signaling, inflammation mediated by chemokine and cytokine signaling, Wnt signaling, and CCKR signaling map. In contrast, P6 proteins were linked to the xanthine and guanine salvage pathway, adenine and hypoxanthine salvage pathway, and blood coagulation pathway. To the best of our knowledge, this study presents the first original perspective on the changes in secretome composition that occur when cAD-MSCs age in vitro. Our findings highlight significant changes that, in conclusion, indicate the regenerative potential of cAD-MSCs and that their secretome may be compromised due to in vitro aging. Consequently, our study suggests a preference for earlier passages when considering these cells for therapeutic applications.

			Action	DRS
	Search.zip	Other
	ZA082TQ_1.raw	Raw
	ZA082TQ_10.raw	Raw
	ZA082TQ_11.raw	Raw
	ZA082TQ_12.raw	Raw

Dataset Information

The role of DENND10 in neuronal development

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets