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The Arabidopsis tyrosine phosphatase RLPH2 targets D-group MAP kinases

ABSTRACT: Knowing that biochemically RLPH2 is a tyrosine phosphatase, we selectively In an attempt to discover RLPH2 substrates we decided to use the power of phospho-proteomics. Immunoprecipitated tyrosine phosphorylated peptides from enriched phospho-peptide pools derived from these plant extracts. Mass spectrometry analysis revealed a dramatic enrichment of tyrosine phosphorylated peptides derived from the activation loops of a subset of Arabidopsis mitogen activated protein kinases (MPKs), specifically the D-group enzymes.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Richard Uhrig

LAB HEAD: Richard Glen Uhrig

PROVIDER: PXD038689 | Pride | 2026-01-26

REPOSITORIES: Pride

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			Action	DRS
	SupplementalTable1_11NOV2022_FINAL.xlsx	Xlsx
	WT_Col_0_rep1.raw	Raw
	WT_Col_0_rep2.raw	Raw
	atrlph2_1_rep1.raw	Raw
	atrlph2_1_rep2.raw	Raw

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Publications

Phospho-Proteomics Identifies D-Group MAP Kinases as Substrates of the Arabidopsis Tyrosine Phosphatase RLPH2.

Labandera Anne-Marie AM Toth Ryan R Mitchell Sierra S Johnson Jayde J JJ Kurucz Brooklyn B Puyaubert Juliette J Baudouin Emmanuel E Uhrig R Glen RG Moorhead Greg B GB

Plant direct 20260120 1

Despite being one of the few bona fide plant tyrosine phosphatases, the Arabidopsis thaliana Rhizobiales-like phosphatase 2 (RLPH2) has no known substrates. Utilizing phospho-proteomics, we identified the activation loop phospho-tyrosine of several A. thaliana D-group mitogen-activated protein kinases (MPKs) as potential RLPH2 substrates. All Arabidopsis D-group MPKs possess a TDY activation loop phosphorylation motif, whereas other MPKs (Groups A, B, and C) contain a ...[more]

PMID: 41567881

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