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Transcriptome-wide Identification of Single-stranded RNA Binding Proteins

ABSTRACT: sinle-stranded RNA binding proteins were identified using DIA MS with the asist of RNA seconderay structure specific probe

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: 瑞奇赵

LAB HEAD: Xiaocheng Weng

PROVIDER: PXD038757 | Pride | 2023-05-10

REPOSITORIES: Pride

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Transcriptome-wide identification of single-stranded RNA binding proteins.

Zhao Ruiqi R Fang Xin X Mai Zhibiao Z Chen Xi X Mo Jing J Lin Yingying Y Xiao Rui R Bao Xichen X Weng Xiaocheng X Zhou Xiang X

Chemical science 20230324 15

RNA-protein interactions are precisely regulated by RNA secondary structures in various biological processes. Large-scale identification of proteins that interact with particular RNA structure is important to the RBPome. Herein, a kethoxal assisted single-stranded RNA interactome capture (KASRIC) strategy was developed to globally identify single-stranded RNA binding proteins (ssRBPs). This approach combines RNA secondary structure probing technology with the conventional method of RNA-binding p ...[more]

PMID: 37063799

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