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Elucidating the RNA-protein Interactomes of Target RNAs in Tissue

ABSTRACT: Raw mass spec files for the protein interactomes of MALAT1, polyadenylated RNA, and scrambled controls in mouse liver and patient derived prostate tumor xenografts XG147 and XG77 as determined by HyPR-MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

DISEASE(S): Prostate Cancer

SUBMITTER: Isabella Whitworth

LAB HEAD: Lloyd M Smith

PROVIDER: PXD038809 | Pride | 2023-07-30

REPOSITORIES: Pride

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Elucidating the RNA-Protein Interactomes of Target RNAs in Tissue.

Whitworth Isabella T IT Henke Katherine B KB Yang Bing B Scalf Mark M Frey Brian L BL Jarrard David F DF Smith Lloyd M LM

Analytical chemistry 20230424 18

RNA-protein interactions are key to many aspects of cellular homeostasis and their identification is important to understanding cellular function. Multiple strategies have been developed for the RNA-centric characterization of RNA-protein complexes. However, these studies have all been done in immortalized cell lines that do not capture the complexity of heterogeneous tissue samples. Here, we develop hybridization purification of RNA-protein complexes followed by mass spectrometry (HyPR-MS) for ...[more]

PMID: 37093976

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