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SW480 N-terminal peptide analysis

ABSTRACT: N-terminal peptide analysis of SW480 cell line for Nt-formylation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Shinyeong Ju

LAB HEAD: Cheolju Lee

PROVIDER: PXD039053 | Pride | 2023-07-20

REPOSITORIES: Pride

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Detection of Nα-terminally formylated native proteins by a pan-N-formyl methionine-specific antibody.

Kim Dasom D Seok Ok-Hee OH Ju Shinyeong S Kim Sang-Yoon SY Kim Jeong-Mok JM Lee Cheolju C Hwang Cheol-Sang CS

The Journal of biological chemistry 20230327 5

N-formyl methionine (fMet)-containing proteins are produced in bacteria, eukaryotic organelles mitochondria and plastids, and even in cytosol. However, Nα-terminally formylated proteins have been poorly characterized because of the lack of appropriate tools to detect fMet independently of downstream proximal sequences. Using a fMet-Gly-Ser-Gly-Cys peptide as an antigen, we generated a pan-fMet-specific rabbit polyclonal antibody called anti-fMet. The raised anti-fMet recognized universally and s ...[more]

PMID: 36990220

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