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Proteome analysis of contrasting rice genotypes under phosphorus-starvation stress

ABSTRACT: This study aims to deal with a comparative proteome analysis of shoot and root tissues of contrasting phosphorus (P) responsive rice genotypes (Pusa-44 and its near-isogenic line (NIL)-23 harboring Pup1 QTL). Proteins were isolated from shoot and root tissues collected from 45-day-old rice plants grown hydroponically in PusaRicH medium with P (16ppm, +P) or without P (0 ppm, -P) under controlled environmental conditions. Following protein quantification using the Bradford method, and quality check using 1D SDS-PAGE, trypsin was used for in-solution digestion and Nano ACQUITY UPLC-MS/MS was used to separate and identify peptides. This study aimed at deciphering the molecular aspect of Pup1 QTL in P-starvation stress tolerance in rice.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Root, Leaf

SUBMITTER: Suresh Kumar

LAB HEAD: Dr. Suresh Kumar

PROVIDER: PXD039084 | Pride | 2023-02-27

REPOSITORIES: Pride

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Proteome analysis of contrasting rice genotypes under phosphorus-starvation stress

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