Proteomics

Dataset Information

0

Identification of gasderminD interacting proteins by mass spectrometry

ABSTRACT: Gasdermin D (GSDMD)-mediated macrophage pyroptosis plays a critical role in inflammation and host defense. Plasma membrane perforation elicited by caspase-cleaved GSDMD N-terminal domain (GSDMD-NT) triggers membrane rupture and subsequent pyroptotic cell death, resulting in release of pro-inflammatory IL-1β and IL-18. Here, using a proteomics approach, we identified fatty acid synthase (FASN) as a GSDMD-binding partner and demonstrated that post-translational palmitoylation of GSDMD at Cys191/Cys192 (human/mouse) led to membrane translocation of GSDMD-NT but not full-length GSDMD. Collectively, we establish GSDMD-NT palmitoylation as a key regulatory mechanism modulating immune activity in infectious and inflammatory diseases.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Arumugam Balasubramanian  

LAB HEAD: Hongbo R Luo

PROVIDER: PXD039133 | Pride | 2025-05-06

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
200626L_ABSAM06877_GSDMDFL2-CTK.XLS.xlsx Xlsx
200626L_ABSAM06877_GSDMDFL2-CTK.mgf Mgf
200626L_ABSAM06877_GSDMDFL2-CTK.raw Raw
200626L_ABSAM06877_GSDMDFL2-CTK_ID_Human_Arumugam.mzML Mzml
200626L_ABSAM06877_GSDMDFL2-CTK_ID_Human_Arumugam.mzid.gz Mzid
Items per page:
1 - 5 of 7
altmetric image

Publications

Plasma membrane perforation elicited by caspase cleavage of the gasdermin D (GSDMD) N-terminal domain (GSDMD-NT) triggers pyroptosis. The mechanisms underlying GSDMD membrane translocation and pore formation are not fully understood. Here, using a proteomic approach, we identified fatty acid synthase (FASN) as a GSDMD-binding partner. S-palmitoylation of GSDMD at Cys<sup>191</sup>/Cys<sup>192</sup> (human/mouse), catalyzed by palmitoyl acyltransferases ZDHHC5 and ZDHHC9 and facilitated by reacti  ...[more]

Similar Datasets

Transcriptomics A palmitoylation-depalmitoylation relay spatiotemporally controls GSDMD activation in pyroptosis [II]
2024-02-25 | GSE252911 | GEO
Transcriptomics A palmitoylation-depalmitoylation relay spatiotemporally controls GSDMD activation in pyroptosis [I]
2024-02-25 | GSE252893 | GEO
Transcriptomics Differences in gene expression between WT and IRF2 KO cells
2019-05-21 | GSE130195 | GEO
Transcriptomics The Card19 locus regulates apoptosis and pyroptosis downstream of caspase activation and gasdermin D cleavage independently from IL-1 cytokine release
2021-03-21 | GSE168489 | GEO
Transcriptomics Vesicular transplantation of gasdermin pores propagates pyroptosis
2025-01-07 | GSE277224 | GEO
Other Control of gasdermin D oligomerization and pyroptosis by the Ragulator-Rag-mTORC1 pathway
2021-06-29 | GSE179089 | GEO
Transcriptomics A bleomycin induced murine model of scleroderma in GSDMD-/- versus wild type
2022-02-16 | GSE196637 | GEO
Proteomics Gasdermin D mediates host cell death but not interleukin-1β secretion in Mycobacterium tuberculosis infected macrophages
2022-02-17 | PXD029017 | Pride
Transcriptomics Effect of genetic deletion of Gsdmd on mice fed an oxalate rich-diet
2025-04-01 | GSE279923 | GEO
Transcriptomics Microglial NLRP3-gasdermin D activation impairs blood-brain barrier integrity through interleukin-1β-independent neutrophil chemotaxis upon peripheral inflammation
2024-12-30 | GSE263094 | GEO