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Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing

ABSTRACT: Structures of RNA editing substrate binding complexes reveal the mechanisms of guide RNA stabilization and messenger RNA recognition in the Trypanosoma brucei mitochondrion.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Trypanosoma Brucei

SUBMITTER: Clinton Yu

LAB HEAD: Z. Hong Zhou

PROVIDER: PXD039156 | Pride | 2024-01-26

REPOSITORIES: Pride

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Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing.

Liu Shiheng S Wang Hong H Li Xiaorun X Zhang Fan F Lee Jane K J JKJ Li Zihang Z Yu Clinton C Hu Jason J JJ Zhao Xiaojing X Suematsu Takuma T Alvarez-Cabrera Ana L AL Liu Qiushi Q Zhang Liye L Huang Lan L Aphasizheva Inna I Aphasizhev Ruslan R Zhou Z Hong ZH

Science (New York, N.Y.) 20230707 6653

In <i>Trypanosoma brucei</i>, the editosome, composed of RNA-editing substrate-binding complex (RESC) and RNA-editing catalytic complex (RECC), orchestrates guide RNA (gRNA)-programmed editing to recode cryptic mitochondrial transcripts into messenger RNAs (mRNAs). The mechanism of information transfer from gRNA to mRNA is unclear owing to a lack of high-resolution structures for these complexes. With cryo-electron microscopy and functional studies, we have captured gRNA-stabilizing RESC-A and g ...[more]

PMID: 37410820

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