Proteomics

Dataset Information

0

BRCA1/BARD1 ubiquitinates PCNA in unperturbed conditions to promote continuous DNA synthesis.


ABSTRACT: Deficiencies in the BRCA1 tumor suppressor gene are the main cause of hereditary breast and ovarian cancer. BRCA1 is involved in the Homologous Recombination DNA repair pathway, and, together with BARD1, forms a heterodimer with ubiquitin E3 activity. The relevance of the BRCA1/BARD1 ubiquitin E3 activity for tumor suppression and DNA repair remains controversial and most efforts aimed to identify BRCA1/BARD1 ubiquitination substrates rely on indirect evidence. Here, we observed that the BRCA1/BARD1 ubiquitin E3 activity was not required for Homologous Recombination or resistance to Olaparib. Using TULIP2 methodology, which enables the direct identification of E3-specific ubiquitination substrates, we identified substrates for BRCA1/BARD1. PCNA is ubiquitinated by BRCA1/BARD1 in unperturbed conditions independently of RAD18, avoiding the formation of ssDNA gaps during DNA replication and promoting replication fork stability upon replication stress, solving the controversy about the function of BRCA1/BARD1 E3 activity in Homologous Recombination.

INSTRUMENT(S): Orbitrap Exploris 480, Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Román González-Prieto  

LAB HEAD: Román González-Prieto

PROVIDER: PXD039167 | Pride | 2024-05-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
E20202001075a.raw Raw
E20202001076a.raw Raw
E20202001077a.raw Raw
E20202001078a.raw Raw
E20202001079a.raw Raw
Items per page:
1 - 5 of 84

Similar Datasets

2019-11-15 | PXD015437 | Pride
2023-08-03 | PXD038326 | Pride
2024-05-31 | PXD047332 | Pride
2023-04-12 | PXD026054 | Pride
2024-05-07 | PXD050319 | Pride
2015-04-29 | E-GEOD-62102 | biostudies-arrayexpress
2023-06-21 | PXD035345 | Pride
2011-08-25 | E-GEOD-31673 | biostudies-arrayexpress
2021-01-29 | PXD013844 | Pride
2011-08-26 | GSE31673 | GEO