Evaluation of depletion of abundant plasma proteins for proteomics characterization of EVs

ABSTRACT: In this study, we aimed to investigate if the removal of abundant plasma proteins prior to EV isolation could improve plasma-derived EV characterization by LC-MS/MS by improving the proteome coverage. Plasma depletion was performed using single-use spin columns prior to SEC-based EV isolation. Afterwards, EVs derived from undepleted and depleted plasma were characterized by mass spectrometry (MS)-based proteomics using a data-independent acquisition (DIA) approach.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Sandrine Reymond  

LAB HEAD: Jean-Charles Sanchez

PROVIDER: PXD039240 | Pride | 2023-07-20

REPOSITORIES: Pride