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Effect of deletion of htpG on global proteomic profile of M. tuberculosis H37Rv

ABSTRACT: The main objective of this study is to understand the effect of deletion of htpG on protein expression profile of M. tuberculosis H37Rv. Whole cell lysates were prepared from 4 biological replicates of the wild-type control and the ∆htpG knockout strains by bead beating in 1X PBS containing protease inhibitors and subjected to 8-plex iTRAQ.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mycobacterium Tuberculosis H37rv

SUBMITTER: Nisheeth Agarwal

LAB HEAD: Nisheeth Agarwal

PROVIDER: PXD039393 | Pride | 2023-07-20

REPOSITORIES: Pride

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HtpG Is a Metal-Dependent Chaperone Which Assists the DnaK/DnaJ/GrpE Chaperone System of Mycobacterium tuberculosis via Direct Association with DnaJ2.

Mangla Nikita N Singh Ramandeep R Agarwal Nisheeth N

Microbiology spectrum 20230406 3

Protein folding is a crucial process in maintaining protein homeostasis, also known as proteostasis, in the cell. The requirement for the assistance of molecular chaperones in the appropriate folding of several proteins has already called into question the previously held view of spontaneous protein folding. These chaperones are highly ubiquitous cellular proteins, which not only help in mediating the proper folding of other nascent polypeptides but are also involved in refolding of the misfolde ...[more]

PMID: 37022172

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