Proteomics

Dataset Information

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Effect of cryopreservation of lysate/tissue on EOC tissue-derived EVs


ABSTRACT: We optimized a method previously developed to extract EVs from epithelial ovarian cancer (EOC) tissues and investigated whether cryopreservation of EOC tissues in clinical practice affects the phenotypes, contents, and biological functions of subsequently extracted EVs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Oocyte

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Shizhen Shen  

LAB HEAD: Shizhen Shen

PROVIDER: PXD039422 | Pride | 2023-05-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
DD079LQ_CA.raw Raw
DD079LQ_CB.raw Raw
DD079LQ_CC.raw Raw
SD343LQB2_MA.raw Raw
SD343LQB2_MB.raw Raw
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Publications

Effects of lysate/tissue storage at -80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins.

Shen Shizhen S   Shen Zhangjin Z   Wang Conghui C   Wu Xiaodong X   Wang Lingfang L   Ye Lele L   Zhang Songfa S   Cheng Xiaodong X  

iScience 20230331 4


Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication. We optimized a method that extracts EVs from epithelial ovarian cancer (EOC) tissues for the purpose of investigating whether cryopreservation of EOC tissues affects the phenotypes, contents, and biological functions of extracted EVs. EV morphology, the number and size distribution of EVs, and EV-related markers were analyzed. Storage of lysates at -80°C decreased lEV yie  ...[more]

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