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Biotinylated H3K9me2 and H3S10ph peptides pull down analysis

ABSTRACT: To further explore the interacting proteins associated with histone modifications, we synthesized biotin labelled peptides for pull down examination.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: xiangzheng zhang

LAB HEAD: xuejiang guo

PROVIDER: PXD039496 | Pride | 2023-03-11

REPOSITORIES: Pride

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Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In Vitro.

Liu Li L Li Haojie H Wang Mengjie M Zhang Xiangzheng X Ren Jie J Yuan Yan Y Sha Jiahao J Guo Xuejiang X

International journal of molecular sciences 20230207 4

Epigenetic regulation, particularly post-translational modifications (PTMs) of histones, participates in spermatogonial stem cell (SSCs) differentiation. However, there is a lack of systemic studies of histone PTM regulation during the differentiation of SSCs due to its low number in vivo. Herein, we quantified dynamic changes of 46 different PTMs on histone H3.1 by targeted quantitative proteomics using mass spectrometry during SSCs differentiation in vitro, in combination with our RNA-seq data ...[more]

PMID: 36834727

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