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Proteasomes of autophagy-deficient cells exhibit changes in associated regulatory proteins in conjunction with a dramatic decrease in activity

ABSTRACT: To identify possible differences in proteasome-associated proteins, we generated AX2 wild-type and ATG16¯ cells expressing the GFP-tagged 20S proteasomal subunit PSMA4 and performed co-immunoprecipitation experiments followed by mass spectrometric analysis.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

SUBMITTER: Prerana Wagle

LAB HEAD: Ludwig Eichinger

PROVIDER: PXD039713 | Pride | 2023-07-20

REPOSITORIES: Pride

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Proteasomes of Autophagy-Deficient Cells Exhibit Alterations in Regulatory Proteins and a Marked Reduction in Activity.

Xiong Qiuhong Q Feng Rong R Fischer Sarah S Karow Malte M Stumpf Maria M Meßling Susanne S Nitz Leonie L Müller Stefan S Clemen Christoph S CS Song Ning N Li Ping P Wu Changxin C Eichinger Ludwig L

Cells 20230530 11

Autophagy and the ubiquitin proteasome system are the two major processes for the clearance and recycling of proteins and organelles in eukaryotic cells. Evidence is accumulating that there is extensive crosstalk between the two pathways, but the underlying mechanisms are still unclear. We previously found that autophagy 9 (ATG9) and 16 (ATG16) proteins are crucial for full proteasomal activity in the unicellular amoeba <i>Dictyostelium discoideum</i>. In comparison to AX2 wild-type cells, ATG9< ...[more]

PMID: 37296637

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