Dataset Information

Hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes

ABSTRACT: Hormonal protocols for cervix dilation are essential for non-surgical embryo recovery (NSER) in ewes, due to the cervical complexity of the species. However, little is known about the possible effects of these hormones on the uterine environment. Thus, this study aimed to evaluate in sheep the effect of a hormonal protocol for cervical dilation on the protein profile of the uterine fluid. After superovulation, Santa Inês ewes (n = 40) were allocated into either a treated group with estradiol benzoate (100 µg, iv) and sodium cloprostenol (0.12 mg, im) 12 h before the embryo collection plus oxytocin (100 IU, iv) 15 min before the collection procedure; or a control group, which consisted of the administration of saline solution. The recovery of the uterine lavage and embryos was performed by laparotomy. After precipitation (trichloroacetic acid) and digestion (trypsin), proteins were identified by mass spectrometry (LC-MS/MS). Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 16 proteins were highly expressed in the treated group (P < 0.05), which included glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundantly in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection.

INSTRUMENT(S):

ORGANISM(S): Ovis Aries

TISSUE(S): Uterine Fluid

SUBMITTER: Proteomics Unit

LAB HEAD: Fábio César Sousa Nogueira

PROVIDER: PXD039757 | Pride | 2025-11-14

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Sheep_v1.msf	Msf
	VL_0008219_CONT87_Sheep_1.raw	Raw
	VL_0008220_CONT87_Sheep_2.raw	Raw
	VL_0008222_CONT75_Sheep_1.raw	Raw
	VL_0008223_CONT75_Sheep_2.raw	Raw

Items per page:

1 - 5 of 42

Similar Datasets

Project description:The prevalence of obesity has significantly increased worldwide. Therefore, this study aimed to evaluate the influence of obesity in the proteomic profile of periodontal ligament (PDL) tissues of rat first maxillary molars submitted to orthodontic tooth movement. The study was approved by the Ethical Committee on Animal Experimentation of the Sao Paulo State University, School of Dentistry at Araraquara, Brazil (protocol number 16/2015) and followed the Animal Research: Reporting of In Vivo Experiments guidelines. Therefore, for this study, we used ten male adult Holtzman rats (weight average – 300 g, approximately 8 weeks old). Animals were randomly assigned to one of the following experimental groups: 1 – M group: animals received a standard diet and 75 days after that, the first maxillary molar was randomly selected to be submitted to OTM for 15 days (n=5), and 2 – OM group: animals received a high-fat diet to induce obesity, and 75 days after that the first maxillary molar was randomly selected to be subjected to OTM for 15 days (n=5). All animals were kept under controlled temperature (22-25 °C), receiving water ad libitum with a 12-hour light/dark cycle. To induce obesity, animals of the OM group received a high-fat diet. In contrast to the standard diet (Labina/Purina, Ribeirão Preto, Brazil) administered to the M group. The high-fat diet contained around 3.82 kcal/g and was composed of 27.5 % of standard rat chow plus 25 % of peanuts, 25 % of milk chocolate, and 12.5 % sweet biscuits and contained 20 g of protein, 48 g of carbohydrate, 20 g of total fat, and 4 g of fiber per 100 g of diet. This feed was daily replaced and had started 75 days before baseline (M initiation). The procedure to induce OTM was by a closed coil nickel-titanium spring (Sentalloy, GAC Central Slip, New York, EUA) connected the maxillary first molar and central incisors with a steel wire (CrNi, 0.20 mm, 55.01.208; Morelli, Sorocaba, Brazi). Fifteen days after OTM, animals were euthanized by anesthetic overdose.

Project description:Prior to pregnancy, hormonal changes lead to cellular adaptations in the endometrium allowing for embryo implantation. Critical for successful pregnancy establishment, innate immune cells constitute a significant proportion of uterine cells prior to arrival of the embryo and throughout the first trimester in humans and animal models. Abnormal uterine immune cell function during implantation is believed to play a role in multiple adverse pregnancy outcomes. Current work in humans has focused on uterine immune cells present after pregnancy establishment, and limited in vitro models exist to explore unique functions of these cells. With single-cell RNA-sequencing (scRNAseq), we comprehensively compared the human uterine immune landscape of the endometrium during the window of implantation and the decidua during the first trimester of pregnancy. We uncovered global and cell-type-specific gene signatures for each timepoint. Immune cells in the endometrium prior to implantation expressed genes associated with immune metabolism, division, and activation. In contrast, we observed widespread interferon signaling during the first trimester of pregnancy. We also provide evidence of specific inflammatory pathways enriched in pre- and post-implantation macrophages and natural killer (NK) cells in the uterine lining. Using our novel implantation-on-a-chip (IOC) to model human implantation ex vivo, we demonstrate for the first time that uterine macrophages strongly promote invasion of extravillous trophoblasts (EVTs), a process essential for pregnancy establishment. Pre- and post-implantation uterine macrophages promoted EVT invasion to a similar degree as pre- and post-implantation NK cells on the IOC. This work provides a foundation for further investigation of the individual roles of uterine immune cell subtypes present prior to embryo implantation and during early pregnancy, which will be critical for our understanding of pregnancy complications associated with abnormal trophoblast invasion and placentation.

Dataset Information

Hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets