Dataset Information

Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes

ABSTRACT: HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid complexes were then analyzed by LC-MS/MS to identify proteins interacting with sphingosine and cholesterol, respectively.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Frank Stein

LAB HEAD: Dr. Doris Hoeglinger

PROVIDER: PXD039780 | Pride | 2023-02-07

REPOSITORIES: Pride

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Publications

Lysosome-targeted multifunctional lipid probes reveal the sterol transporter NPC1 as a sphingosine interactor.

Altuzar Janathan J Notbohm Judith J Stein Frank F Haberkant Per P Hempelmann Pia P Heybrock Saskia S Worsch Jutta J Saftig Paul P Höglinger Doris D

Proceedings of the National Academy of Sciences of the United States of America 20230309 11

Lysosomes are catabolic organelles involved in macromolecular digestion, and their dysfunction is associated with pathologies ranging from lysosomal storage disorders to common neurodegenerative diseases, many of which have lipid accumulation phenotypes. The mechanism of lipid efflux from lysosomes is well understood for cholesterol, while the export of other lipids, particularly sphingosine, is less well studied. To overcome this knowledge gap, we have developed functionalized sphingosine and c ...[more]

PMID: 36893262

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Dataset Information

Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes

Publications

Lysosome-targeted multifunctional lipid probes reveal the sterol transporter NPC1 as a sphingosine interactor.

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