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Interaction protoemics of Dictyostelium discoideum GFP-SAC1

ABSTRACT: Label free interaction protoemics of Dictyostelium discoideum GFP-SAC1 compared to GFP expressing cells

INSTRUMENT(S): Q Exactive

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

SUBMITTER: Florian Fröhlich

LAB HEAD: Florian Fröhlich

PROVIDER: PXD039848 | Pride | 2023-12-01

REPOSITORIES: Pride

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Structure of the ceramide-bound SPOTS complex.

Schäfer Jan-Hannes JH Körner Carolin C Esch Bianca M BM Limar Sergej S Parey Kristian K Walter Stefan S Januliene Dovile D Moeller Arne A Fröhlich Florian F

Nature communications 20231004 1

Sphingolipids are structural membrane components that also function in cellular stress responses. The serine palmitoyltransferase (SPT) catalyzes the rate-limiting step in sphingolipid biogenesis. Its activity is tightly regulated through multiple binding partners, including Tsc3, Orm proteins, ceramides, and the phosphatidylinositol-4-phosphate (PI4P) phosphatase Sac1. The structural organization and regulatory mechanisms of this complex are not yet understood. Here, we report the high-resoluti ...[more]

PMID: 37794019

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