Identification of Cross-linking partners of BpA modified p97 by photo cross-linking MS

ABSTRACT: We wanted to see where and how the protein p97 interacts with its partner proteins p37, PPI,Sds22 and I3. To this end a p97 derivative with genetically encoded crosslinking amino acid (p-benzoyl-L-phenylalanine) was generated. After incubation with the partner proteins, photocross-linking and tryptic digest the resulting cross-linked peptides were analysed by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Farnusch Kaschani  

LAB HEAD: Dr Farnusch Kaschani

PROVIDER: PXD039939 | Pride | 2023-07-10

REPOSITORIES: Pride