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The effects of silencing PCYOX1 on the proteome of murine fibroblast cells 3t3-L1 differentiated to adipocyte-like cells

ABSTRACT: In order to evaluate the impact of PCYOX1 deletion on adipogenesis, we applied a label-free mass spectrometry-based proteomic approach to compare the proteome of 3T3-L1 differentiated for 9 days to adipocytes after PCYOX1 gene silencing. Pcyox1 was silenced using shRNA lentiviral particles. Control cells were treated with shRNA lentiviral particles containing a negative construct.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Erica Gianazza

LAB HEAD: Cristina Banfi

PROVIDER: PXD039943 | Pride | 2023-05-10

REPOSITORIES: Pride

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Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis.

Banfi Cristina C Mallia Alice A Ghilardi Stefania S Brioschi Maura M Gianazza Erica E Eligini Sonia S Sahlén Pelin P Baetta Roberta R

Antioxidants (Basel, Switzerland) 20230221 3

The process of adipogenesis involves the differentiation of preadipocytes into mature adipocytes. Excessive adipogenesis promotes obesity, a condition that increasingly threatens global health and contributes to the rapid rise of obesity-related diseases. We have recently shown that prenylcysteine oxidase 1 (PCYOX1) is a regulator of atherosclerosis-disease mechanisms, which acts through mechanisms not exclusively related to its pro-oxidant activity. To address the role of PCYOX1 in the adipogen ...[more]

PMID: 36978789

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