Proximity labeling of TurboID-SPARKEL

ABSTRACT: Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which we term SPARKEL. Here, we performed proximity labeling of parasites expressing TurboID-SPARKEL endogenously.

INSTRUMENT(S):

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen  

LAB HEAD: Sebastian Lourido

PROVIDER: PXD039986 | Pride | 2024-07-12

REPOSITORIES: Pride