Proteomics

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The effects of silencing PCYOX1 on the proteome of murine cardiomyocites HL-1

ABSTRACT: In order to evaluate the impact of PCYOX1 deletion on cardiomyocytes, we applied a label-free mass spectrometry-based proteomic approach to compare the proteome of Hl-1 after PCYOX1 gene silencing, at basal conditions, after hypoxia and after hypoxia/reoxygenation. Pcyox1 was silenced using shRNA lentiviral particles. Control cells were treated with shRNA lentiviral particles containing a negative construct.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Erica Gianazza  

LAB HEAD: Cristina Banfi

PROVIDER: PXD040095 | Pride | 2026-04-27

REPOSITORIES: Pride

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Dataset's files

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Action DRS
HL1_proteome_shPCYOX_2022.DerivedFromSearch879298eb-820d-47eb-8853-7cab788ff365.mgf Mgf
HL1_proteome_shPCYOX_2022.mzid.gz Mzid
IR8_neg_Hypoxia_PROTEOME_240821_01.mzML Mzml
IR8_neg_Hypoxia_PROTEOME_240821_02.mzML Mzml
IR8_neg_Riperfusion_PROTEOME_240821_01.mzML Mzml
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Publications

Prenylcysteine Oxidase 1 Deficiency Protects the Cardiac Muscle Cell Line HL-1 Against Ischaemic/Hypoxic Stress.

Banfi Cristina C   Brocca Lisa L   Bascucci Alice A   Papaianni Giulia Giusy GG   Mallia Alice A   Eligini Sonia S  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20260401 8

Loss of cardiomyocytes during hypoxia-reoxygenation injury contributes to adverse myocardial remodeling, resulting in hypertrophy of surviving cardiomyocytes, interstitial fibrosis, and ultimately, heart dysfunction. Despite extensive research in the field, there is currently no specific treatment available for myocardial hypoxia-reoxygenation injury to prevent cardiomyocyte death. Prenylcysteine oxidase 1 (PCYOX1) is a pro-oxidant, FAD-dependent thioether oxidase that generates hydrogen peroxid  ...[more]

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