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Phycocyanin-Allophycocyanin LC-MSMS

ABSTRACT: In this study, a co-expressed E. coli strain of phycocyanin-allophycocyanin was constructed, and co-expressed with chromophore in E. coli. Different molecular weights of phycocyanin and allophycocyanin were detected in the recombinant strains, indicating that the expressed polymers are different. The 66kDa dimer and the 300kDa multimer contained phycocyanin and allophycocyanin, identified by mass spectrometry.

INSTRUMENT(S): 4700 Proteomics Analyzer

ORGANISM(S): Escherichia Coli

SUBMITTER: Menghui SHANG

LAB HEAD: Xiaonan ZANG

PROVIDER: PXD040154 | Pride | 2023-05-10

REPOSITORIES: Pride

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Fluorescence and antioxidant activity of heterologous expression of phycocyanin and allophycocyanin from <i>Arthrospira platensis</i>.

Shang Meng-Hui MH Sun Jian-Fei JF Bi Ying Y Xu Xiao-Ting XT Zang Xiao-Nan XN

Frontiers in nutrition 20230220

Phycocyanin and allophycocyanin are important active substances in <i>Arthrospira platensis</i>, because of their fluorescent characteristic and antioxidant capacity. In order to solve the problem of insufficient production and inconvenient modification of natural protein, recombinant expression was performed and the fluorescence activity and antioxidant activity was analyzed to meet the demand for phycocyanin and allophycocyanin. A total of seven recombinant strains were constructed in this stu ...[more]

PMID: 36891162

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