Purification of Human β and γ Actin from Budding Yeast

ABSTRACT: To facilitate biochemical studies of human cytoplasmic actins, Saccharomyces cerevisiaestrains were developed that express either human β-actin or γ-actin as their sole actin. Actins purified from these strains have biochemical properties similar to mammalian skeletal muscle α-actin, but display differential binding to some key actin regulators. Mass-spectrometry analysis shows that the human actins made from yeast generally retain their N-terminal methionines, which are modified by acetylation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Oryctolagus Sp. 'rabbit_od' Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture, Femoral Muscle

SUBMITTER: Ebbing de Jong  

LAB HEAD: Jessica Henty-Ridilla

PROVIDER: PXD040174 | Pride | 2023-04-24

REPOSITORIES: Pride