Dataset Information

Expression of spider silk in tobacco improves drought tolerance with minimal effects on its mechanotype

ABSTRACT: Spider silk, especially dragline silk from Trichonephila clavipes, is an excellent natural material with remarkable mechanical properties. In this study, we generated nucleus- and plastid-encoded expression of a partial sequence of one of the components of dragline silk, major ampullate spidroin-1 (MaSp1), in tobacco (MaSp1-tobacco), MaSp1nuc and MaSp1pla, respectively. The partial sequence of MaSp1 encodes six repetitive glycine-rich and polyalanine tandem domains, and we evaluated the effect of its expression on plant mechanical properties and physiology. While MaSp1 expression showed no effect on leaf mechanical properties, it conferred drought tolerance. MaSp1-tobacco demonstrated an increased growth on PEG-infused growth medium and higher drought recovery in soil compared to the non-transgenic control (WT). Transcriptome analyses of drought-stressed MaSp1-tobacco revealed an upregulation of genes involved in stress response, antioxidant activity, and phenylpropanoid biosynthesis, with a higher effect of drought on the MaSp1pla transcriptome. Genes encoding peroxidase, stress and channel proteins, and cellular metabolism and homeostasis were consistently upregulated in these lines, which also displayed a higher total antioxidant status (TAS) compared to WT, and a higher TAS in MaSp1pla compared to MaSp1nuc. Under normal watering conditions, the ABA content and the TAS in MaSp1-tobacco were significantly higher than in the WT. Our results show that nucleus- and plastid-encoded expression of MaSp1 in tobacco increased the endogenous ABA levels, which led to the formation of plant stress memory that caused overall adjustments in multiple drought tolerance mechanisms and hence, conferred drought tolerance.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Leaf

SUBMITTER: Takehiro Suzuki

LAB HEAD: Naoshi Dohmae

PROVIDER: PXD040180 | Pride | 2025-05-06

REPOSITORIES: Pride

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Expression of spider silk protein in tobacco improves drought tolerance with minimal effects on its mechanotype.

Morey-Yagi Shamitha Rao SR Hashida Yoichi Y Okamoto Masanori M Odahara Masaki M Suzuki Takehiro T Thagun Chonprakun C Foong Choon Pin CP Numata Keiji K

The Plant journal : for cell and molecular biology 20250101 2

Spider silk, especially dragline silk from golden silk spiders (Trichonephila clavipes), is an excellent natural material with remarkable mechanical properties. Many studies have focused on the use of plants as biofactories for the production of recombinant spider silk. However, the effects of this material on the mechanical properties or physiology of transgenic plants remain poorly understood. Since glycine-rich proteins play key roles in plants, we evaluated the effects of a glycine-rich spid ...[more]

PMID: 39866095

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			Action	DRS
	MaSp1nucNo1.mzML	Mzml
	MaSp1nucNo1.mzid.gz	Mzid
	MaSp1nucNo1.raw	Raw
	MaSp1plaNo2-2.mzML	Mzml
	MaSp1plaNo2-2.mzid.gz	Mzid