Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: Douglas Lamont
LAB HEAD: Professor Ed Hurt
PROVIDER: PXD040306 | Pride | 2023-10-24
REPOSITORIES: Pride
Castillo Duque de Estrada Nestor Miguel NM Thoms Matthias M Flemming Dirk D Hammaren Henrik M HM Buschauer Robert R Ameismeier Michael M Baßler Jochen J Beck Martin M Beckmann Roland R Hurt Ed E
Nature structural & molecular biology 20230608 8
The 5S ribonucleoprotein (RNP) is assembled from its three components (5S rRNA, Rpl5/uL18 and Rpl11/uL5) before being incorporated into the pre-60S subunit. However, when ribosome synthesis is disturbed, a free 5S RNP can enter the MDM2-p53 pathway to regulate cell cycle and apoptotic signaling. Here we reconstitute and determine the cryo-electron microscopy structure of the conserved hexameric 5S RNP with fungal or human factors. This reveals how the nascent 5S rRNA associates with the initial ...[more]