Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic evaluation of urine of the female of domestic dog (Canis familiaris), during different stages of ovarian cycle

ABSTRACT: Chemical signals are essential for communication between living organisms. Dogs possess two sensory organs enabling chemical communication; the main olfactory system and the vomeronasal organ (VNO). Besides, contact chemoreception is also pertinent, by which non-volatile molecules, including but not limited to proteins, are recognized as chemical signals. However, non-volatile chemical signals have been sparsely studied in dogs. Therefore, we aimed to examine the urinary proteins of female domestic dogs during estrus and anestrus phases to detect and identify such non-volatile chemical signals.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Urine

SUBMITTER: Marcin Skwark

LAB HEAD: Michał Dzięcioł

PROVIDER: PXD040418 | Pride | 2023-05-10

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Urinary Proteins of Female Domestic Dog (<i>Canis familiaris</i>) during Ovarian Cycle.

Woszczyło Martyna M Pasikowski Paweł P Devaraj Sankarganesh S Kokocińska Agata A Szumny Antoni A Skwark Marcin J MJ Niżański Wojciech W Dzięcioł Michał M

Veterinary sciences 20230414 4

The presence and identity of non-volatile chemical signals remain elusive in canines. In this study, we aim to evaluate the urinary proteins of female domestic dogs in the estrus and anestrus phases to evidence the presence of non-volatile chemical signals and to elucidate their identities. We collected urine samples from eight female dogs in the estrus and anestrus phases. A total of 240 proteins were identified in the urine samples using liquid chromatography-mass spectrometry (LC-MS analysis) ...[more]

PMID: 37104448

Similar Datasets

Domestic dog lineages reveal genetic drivers of behavioral diversification

Project description:Selective breeding of domestic dogs has generated diverse breeds often optimized for performing specialized tasks. Despite the heritability of breed-typical behavioral traits, identification of causal loci has proven challenging due to the complexity of canine population structure. We overcome longstanding difficulties in identifying genetic drivers of canine behavior by developing an innovative framework for understanding relationships between breeds and the behaviors that define them utilizing genetic data for over 4,000 domestic, semi-feral and wild canids and behavioral survey data for over 46,000 dogs. We identify ten major canine genetic lineages and their behavioral correlates and show that breed diversification is predominantly driven by non-coding regulatory variation. We determine that lineage-associated genes converge in neurodevelopmental co-expression networks, identifying a sheepdog-associated enrichment for interrelated axon guidance functions. This work presents a scaffold for canine diversification that positions the domestic dog as an unparalleled system for revealing the genetic origins of behavioral diversity.

2022-12-07 | GSE213053 | GEO

Identification of potential chemical signals in the European water vole Arvicola terrestris

Project description:The water vole Arvicola terrestris (= Arvicola amphibious L.) is endemic in Europe where its outbreak generates severe economic losses for farmers. Our project aimed at deciphering the modalities of chemical communication in this species, to develop new sustainable methods for populations control. The water vole, as well as other rodents, uses specific urination sites as territorial and sex pheromone markers, the chemical and biochemical identification of which is still unknown. Lateral scent glands and urine samples were collected from wild males and females caught in the field, at different periods of the year. Volatile signals were searched in urine by SPME/GC-MS, and in lateral scent glands by solvent extraction followed by GC-MS. The volatile composition of urine was analysed for each individual and not on pooled samples, showing no significant difference between males and females. Lateral scent glands contained some volatile components (pyrazines, alcohols, terpenes), and mostly long chain fatty acid esters, again without quantitative and qualitative differences between sexes. Conversely, the urinary protein content, analysed by 1D- and 2D-electrophoresis is different, as only males secrete high levels of lipocalins, whatever the reproduction period. The proteins were identified by mass spectrometry and “de novo” analysis (Orbitrap), which provided specific information to design primers for PCR amplification of cDNA sequences. Thus, urinary proteins were characterized by direct amplification from liver RNA. The identified protein sequences are closed to those of Myodes glareolus, a closely related species of Cricetidae, and to hamster Aphrodisin.

2019-12-11 | PXD013803 | Pride

Scentmarking communication in the female rat_C4PR_LIV

Project description:Chemical communication in mammals is in part mediated through the deposition of scent marks in the environment. Volatile and non-volatile moleculesin the scent marksconvey important information about the donor animal, such as sex, social and reproductive status,and stimulatea response in the receptor animal. In rodents, most of the work about deciphering the molecular composition of scent markshas been focused on mice. In rats, by contrast, knowledge is limitedand the molecular complexity of scent marks is not as well described as in mice. In particular, the sources and roles of proteins in scent marks are largely undefined, with even less knowledge when we consider female rats. We therefore conducted a global proteomic analysis of scent marks from femalerats (Rattus norvegicus)to define the protein composition and then we investigated the role of scent marks in sexual communication. Our study revealed that protein pattern inscent marks greatly differsfrom that in naturally voided urine, indicatinga different tissue of41origin. In particular, female scent marks are dominated by lipocalins, notablymajor urinary proteins, whereas urinary output of these proteins is almost imperceptible.Further analysis revealed that the most plausible source of the components in scent marks are the clitoral glands,implying a critical role of theseaccessory glands in sexual communication in the rat.

2023-05-29 | PXD039325 | Pride

Volatile communication in plants relies on a KAI2-mediated signaling pathway

Project description:Plants are targets of volatile organic compounds (VOCs) released as a part of plant-plant communication, within-plant self-signaling and plant-microbe interactions. Therefore, understanding VOC perception and downstream signaling is vital for unraveling the mechanisms behind information exchange in plants, which remain largely unexplored. Using the hormone-like function of volatile terpenoids in reproductive organ development as a system with a visual marker for communication, we demonstrated that among the four petunia karrikin-insensitive receptors, PhKAI2ia stereo-specifically perceives the (-)-germacrene D signal, triggering a karrikin-like signaling cascade. This study provides new insights into plant olfaction, uncovers the role(s) of the unique intermediate clade of KAI2 receptors, illuminates the involvement of KAI2ia-dependent signaling pathway in volatile communication and gives insight into the long-standing question about the nature of potential endogenous karrikin-like ligand(s).

2024-02-07 | GSE245080 | GEO

Serum proteome profiling across different stages of myxomatous mitral valve disease in domestic dogs

Project description:Development and progression of myxomatous mitral valve disease (MMVD) in domestic dogs is unpredictable and pathobiology still unclear. The American College of Veterinary Internal Medicine (ACVIM) perceived that mayor improvement in management of diseased dogs would be timely diagnosis, especially detection of transition from MMVD stage B1 into B2. Thus, in this study we compared by tandem mass tag (TMT) protocol and mass spectrometry (MS) acquired quantitative proteome profiles of serum collected from healthy (control) (N=12) and dogs diagnosed with different stages of naturally occurring MMVD: B1 (N=13), B2 (N=12) and C (N=13). Prior to proteomic analysis dogs were distinguished into experimental categories based on echocardiography results. Serum biochemistry and concentrations of three cardiac biomarkers (galectin-3, suppression of tumorigenicity 2 and asymmetric dimethylarginine) were performed to obtain better characterization of healthy/control group and MMVD cases.

2023-05-10 | PXD038475 | Pride

Activation of defense pathways in Scots pine bark after feeding by Pine Weevil (Hylobius abietis)

Project description:Coniferous trees use the oleoresin, a mixture of non-volatile non-volatile diterpene acids and a large (20-50%) volatile fraction of mono- and sesquiterpenes, as a defence tool against damaging herbivores and pathogens. Volatilization of monoterpenes increases viscosity of oleoresin and finally leading to polymerization of resin and a formation of protective solid plug. Resin storing conifers form an important source of volatile organic compounds (VOCs) mainly dominated by volatile monoterpenes. In the atmosphere, conifer VOCs react with ozone and OH and nitrous oxide radicals forming secondary organic compounds. This project seeks a better understanding of the genetic control of conifer defences and the role of monoterpenoid compounds under biotic stresses.

2015-04-01 | GSE60383 | GEO

A non-Âinvasive method for sampling the body odour of mammals

Project description:<p>Olfaction is a central aspect of mammalian communication, providing information about individual attributes such as identity, sex, group membership or genetic quality. Yet, the chemical underpinnings of olfactory cues remain little understood, one of the reasons being the difficulty in obtaining high quality samples for chemical analysis. In this study, we adjusted and evaluated the use of thermal desorption (TD) tubes, commonly used in plant metabolomic and environmental studies, for non-invasive sampling of mammalian body odour. We obtained chemical profiles of meerkat (Suricata suricatta) body odour samples, using TD tubes analysed with gas chromatographyâmass spectrometry. TD tubes captured a wide range of volatile and semi-volatile organic compounds, including compounds likely originating from the target animals. Adjustment of sampling parameters (distance, volume, flow rate, interruption of sampling) to increase the feasibility for a non-invasive application yielded samples of adequate quality. However, to minimize the variability between samples, sampling parameters should be kept constant and samples should be collected when no conspecifics are close-by. The method was sensitive enough to pick up population differences in the chemical profiles of two captive groups of meerkats, demonstrating its applicability to biological questions. With sufficiently habituated animals, the method is applicable non-invasively, allowing short- and long-term studies on a wide range of questions, including e.g. chemical signatures of kinship, diet, individual health or reproductive state.</p>

2022-01-14 | MTBLS557 | MetaboLights

Activation of defense pathways in Scots pine bark after feeding by Pine Weevil (Hylobius abietis)

2015-04-01 | E-GEOD-60383 | biostudies-arrayexpress

Streptomyces venezuelae ATCC 10712

Project description:Streptomyces exploration is triggered by fungal interactions and volatile signals

| PRJNA341574 | ENA

Benchmark Dose Modeling Approaches for Volatile Organic Chemicals using a Novel Air-Liquid Interface In Vitro Exposure System

Project description:Inhalation is the most relevant route of volatile organic chemical (VOC) exposure; however, due to unique challenges posed by their chemical properties and poor solubility in aqueous solutions, in vitro chemical safety testing is predominantly performed using direct application dosing/submerged exposures. To address the difficulties in screening toxic effects of VOCs, our cell culture exposure system permits cells to be exposed to multiple concentrations at air-liquid interface (ALI) in a 24-well format. ALI exposure methods permit direct chemical-to-cell interaction with the test article at physiological conditions. In the present study, BEAS-2B and primary normal human bronchial epithelial cells (pHBEC) are used to assess gene expression, cytotoxicity, and cell viability responses to a variety of volatile chemicals including acrolein, formaldehyde, 1,3-butadiene, acetaldehyde, 1-bromopropane, carbon tetrachloride, dichloromethane, and trichloroethylene. BEAS-2B cells were exposed to all the test agents, while pHBECs were only exposed to the latter four listed above. The VOC concentrations tested elicited only slight cell viability changes in both cell types. Gene expression changes were analyzed using benchmark dose (BMD) modeling. The BMD for the most sensitive gene set was within one order of magnitude of the threshold-limit value reported by the American Conference of Governmental Industrial Hygienists, and the most sensitive gene sets impacted by exposure correlate to known adverse health effects recorded in epidemiologic and in vivo exposure studies. Overall, our study outlines a novel in vitro approach for evaluating molecular-based points-of-departure in human airway epithelial cell exposure to volatile chemicals.

2022-04-17 | GSE199794 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data