Proteomics

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New Pathophysiological Insights from Serum Proteome Profiling in Equine Atypical Myopathy.


ABSTRACT: Equine atypical myopathy (AM) is a severe environmental intoxication linked to the ingestion of protoxins contained in seeds and seedlings of the sycamore maple (Acer pseudoplatanus) in Europe. The toxic metabolites cause a frequently fatal rhabdomyolysis syndrome in grazing horses. Since toxic metabolites can also be present in co-grazing horses, it is still unclear as to why, in a similar environmental context, some horses show signs of AM whereas others remain clinically healthy. Label-free proteomics analyses on the serum of 26 diseased AM, 23 co-grazers and 11 control horses were performed to provide insights into biological processes and pathways. A total of 43 and 44 differentially expressed proteins between “AM vs co-grazing horses” and “AM vs control horses” were found. Disease-linked changes in the proteome of different groups were found to correlate with detected amounts of toxins and principal component analysis was performed to identify the 29 proteins with the greatest impact on the proteomic differences between groups. Among the pathway-specific changes the glycolysis/gluconeogenesis pathway, the coagulation/complement cascade, and the biosynthesis of amino acid were affected. Sycamore maple poisoning results in a combination of inflammation, oxidative stress, and high energy demand, which is trying to be met by enhanced glycolysis.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Blood Cell, Blood

DISEASE(S): Myopathy

SUBMITTER: Marc Dieu  

LAB HEAD: Dominique-M. Votion

PROVIDER: PXD040429 | Pride | 2024-02-09

REPOSITORIES: Pride

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