Dataset Information

Lead eliminating activity of Auricularia polytricha glycoprotein protects against lead acetate-induced hepatorenal toxicity in rats via positive immunoregulation pathway

ABSTRACT: Lead is a ubiquitous environmental and industrial pollutant. Its nonbiodegradable toxicity induces a plethora of human diseases. The current study was undertaken to purify a novel 252-kDa bioactive glycoprotein containing 1.15% carbohydrate from the edible fungus Auricularia polytricha with the ability of adsorbing lead and producing detoxification. The A. polytricha detoxifying glycoprotein (APL), which displayed unique molecular properties, was purified by ion exchange chromatography and gel filtration chromatography. For investigating the protective effects of A. polytricha, Sprague Dawley (SD) rats were divided into six groups which received daily intraperitoneal injection of lead acetate for 30 days, followed by gavage with APL (40, 80, 160 mg/kg B body weight) and EDTA (300 mg/kg body weight) for 30 days after successful establishment of an animal model of lead detoxification. The serum concentrations of lead and the liver biomarkers AST and ALT were significantly (p<0.05) improved by APL treatments, as well as treatment with the positive control EDTA. Likewise, results on lead residue showed that the clearance ratios of liver and kidney were respectively 44.5% and 18.1% at the high APL dosage, which was even better than the corresponding data for EDTA. Proteomics disclosed that 351 proteins differentially expressed due to lead exposure and the expression levels of 41 proteins enriched in the pathways mainly involved in cell detoxification and immune regulation were normalized after treatment with APL-H. Our results signify that APL may ameliorate lead-induced hepatorenal injury by positive regulation of immune processing, and suggest that APL be applied as a therapeutic intervention of lead poisoning in clinic treatment. This report represents the first demonstration of the protective action of a novel mushroom protein on lead-elicited hepatorenal toxicity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Liver

SUBMITTER: Shuang Zhao

LAB HEAD: Shuang Zhao

PROVIDER: PXD040493 | Pride | 2023-04-04

REPOSITORIES: Pride

ACCESS DATA

Similar Datasets

Project description:Polycyclic aromatic hydrocarbons are common pollutants in soil, have negative effects on soil ecosystems, and are potentially carcinogenic. The Springtail (Collembola) Folsomia candida is often used as an indicator species for soil toxicity. Here we report a toxicogenomic study that translates the ecological effects of the polycyclic aromatic hydrocarbon phenanthrene in soil to the early transcriptomic responses in Folsomia candida. Microarrays were used to examine two different exposure concentrations of phenanthrene, namely the EC10 (24.95 mg kg-1 soil) and EC50 (45.80 mg kg-1 soil) on reproduction of this springtail, which evoked 405 and 251 differentially expressed transcripts, respectively. Fifty transcripts were differential in response to either concentration. Many transcripts encoding xenobiotic detoxification and biotransformation enzymes (phases I, II, and III) were upregulated in response to either concentration. Furthermore, indications of general and oxidative stress were found in response to phenanthrene. Chitin metabolism appeared to be disrupted particularly at the low concentration, and protein translation appeared suppressed at the high concentration of phenanthrene; most likely in order to reallocate energy budgets for the detoxification process. Finally, an immune response was evoked especially in response to the high effect concentration, which was also described in a previous transcriptomic study using the same effect concentration (EC50) of cadmium. Our study provides new insights in the molecular mode of action of the important polluting class of polycyclic aromatic hydrocarbons in soil animals. Furthermore, we present a fast, sensitive, and specific soil toxicity test which enhances traditional tests and may help to improve current environmental risk assessments and monitoring of potentially polluted sites. Folsomia candida was exposed to phenanthrene spiked soil or untreated (reference/control) soil for 2 days. Two different concentrations of phenanthrene were used, 24.95 and 45.80 mg/kg soil which represent the EC10 and EC50 on reproduction, respectively. For each concentration treatment 4 biological replicates were used, replicate samples consisted of total RNA extracted from ~30 animals exposed in the same jar to either reference or phenanthrene spiked soil. Phenanthrene treated samples were always hybridized to reference samples in an evenly distributed dye-swap manner, which resulted in total in 8 hybridizations of 16 samples.

Dataset Information

Lead eliminating activity of Auricularia polytricha glycoprotein protects against lead acetate-induced hepatorenal toxicity in rats via positive immunoregulation pathway

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure