Potential of Negative Ion Mode Proteomics: MS1-Only Approach

ABSTRACT: Advancing Negative Ion Mode Proteomics. The main objective of the project is the exploration of the unconvetional negative ion mode for proteomics studies. In this work, we thoroughly studied the best chromatographic conditions for negative ion mode proteomics before testing different enzymatic digestion. The final goal is to establish the best working conditions in the negative polarity for negative ion mode. The method also refrains from any fragmentation events, which are unpredictable in negative ion mode.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Pelayo Alvarez Penanes  

LAB HEAD: Frank Kjeldsen

PROVIDER: PXD040583 | Pride | 2023-07-20

REPOSITORIES: Pride