Dataset Information

Proteomes of individuals of Eulimnogammarus verrucosus collected in the field in fall-winter months

ABSTRACT: Eulimnogammarus verrucosus is a Baikal endemic species inhabiting the lake's littoral. This species is adapted to low temperatures and it reproduces during fall-winter months. To capture aspects of adaptation to winter conditions in situ, we evaluated changes in the proteomes of E. verrucosus that were collected during the fall-winter months (September, November, December, and January) and early summer (June). Moreover, the aspects of reproduction of the cold-reproducing species were traced.

INSTRUMENT(S):

ORGANISM(S): Eulimnogammarus Verrucosus

TISSUE(S): Whole Body

SUBMITTER: Polina Lipaeva

LAB HEAD: Till Luckenbach

PROVIDER: PXD040677 | Pride | 2025-07-28

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 148

Publications

How to Reproduce in the Siberian Winter: Proteome Dynamics Reveals the Timing of Reproduction-Related Processes in an Amphipod Species Endemic to Lake Baikal.

Lipaeva Polina P Drozdova Polina P Vereshchagina Kseniya K Jakob Lena L Schubert Kristin K Bedulina Daria D Luckenbach Till T

Ecology and evolution 20250723 7

The winters in the region of the vast global freshwater biodiversity hotspot Lake Baikal are extremely cold. Although the conditions for reproduction may seem unfavorable during winter, the lake is inhabited by a major endemic winter-reproducing amphipod species complex. Compared with Baikal's summer-reproducing amphipod species, the duration of a reproduction cycle in the winter-reproducing species is more extended. We hence hypothesized that in those species, reproduction-related processes dep ...[more]

PMID: 40703971

Similar Datasets

Project description:Winter survival and maintenance strategy is crucial in temperate woody plants. Here, we demonstrate novel aspects of the transcriptional regulations adopted by perennial tree species in winter/dormancy, employing a biochemical and whole transcriptome analysis. As expected, genes related to cold hardiness and defense are over-represented. Interestingly, carbohydrate biosynthesis and transport-related genes were very actively expressed in winter/dormancy. Further biochemical analyses verified the dormancy/winter transcription phenotype. Furthermore, dormancy/winter preferential expression of genes involved in the cell wall biosynthesis/modification, circadian rhythm, the indirect transcriptional regulation (RNA metabolism), and chromatin modification/remodeling were identified. Taken together, regulation of gene expression in the winter survival and maintenance may include not only controlled by promoter binding transcription factors but may also be regulated at the post-transcriptional and chromatin levels. In the first step towards the understanding of molecular mechanisms underlying the winter survival and maintenance of perennial trees, we examined the characteristics of transcription phenotype of the dormancy/winter stem compared to the active growth/summer stem by using a whole transcriptome analysis (GeneChip Poplar Genome Array; Affymetrix). Poplar Genome Array has a total of 61,251 probe sets, representing 57,423 poplar gene models, and allows us to interrogate a total of 41,558 unique gene models because of the probe set redundancy. Slight redundancy of the probe sets within a single chip gave us a unique opportunity to have internal comparisons of the particular genes. Very high reproducibility (R2 > 0.97) between the replicated samples was found. Further confirmation of the GeneChip data was made by semi-quantitative RT-PCR analysis using several genes showing the summer or winter biased expression. Based on these results, further analysis of winter stem transcriptome against summer stem was performed.

			Action	DRS
	Field_Sample10_f1.raw	Raw
	Field_Sample10_f2.raw	Raw
	Field_Sample11_f1.raw	Raw
	Field_Sample11_f2.raw	Raw
	Field_Sample12_f1.raw	Raw