Name: cryo-EM study of human liver cell lysate
Keywords: Human liver

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Cryo-EM study of human liver cell lysate

ABSTRACT: This is the proteomic analysis data of Human liver cell lysate subjected to cryo-EM study.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: zhemin zhang

LAB HEAD: Edward W.

PROVIDER: PXD040733 | Pride | 2024-01-26

REPOSITORIES: Pride

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High-resolution structural-omics of human liver enzymes.

Su Chih-Chia CC Lyu Meinan M Zhang Zhemin Z Miyagi Masaru M Huang Wei W Taylor Derek J DJ Yu Edward W EW

Cell reports 20230607 6

We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) to define its composition. From this sample we identified and simultaneously determined high-resolution structural information for ten unique human liver enzymes involved in diverse cellular processes. Notably, we determined the structure of the endoplasmic bifunctional protein H6PD, where the N- and C-terminal domains independently possess glucose-6-phosphate dehydrogenase and 6-phosph ...[more]

PMID: 37289586

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