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Protein content and proximity map of yeast nuclear mRNPs

ABSTRACT: We isolated nuclear mRNPs from yeast using an endogenous bi-molecular tagging approach on Sub2 and Hpr1.

INSTRUMENT(S): Orbitrap Exploris 480, Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Barbara Steigenberger

LAB HEAD: Elena Conti

PROVIDER: PXD040736 | Pride | 2023-07-25

REPOSITORIES: Pride

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Nuclear mRNPs are compact particles packaged with a network of proteins promoting RNA-RNA interactions.

Bonneau Fabien F Basquin Jérôme J Steigenberger Barbara B Schäfer Tillman T Schäfer Ingmar B IB Conti Elena E

Genes & development 20230630 11-12

Messenger RNAs (mRNAs) are at the center of the central dogma of molecular biology. In eukaryotic cells, these long ribonucleic acid polymers do not exist as naked transcripts; rather, they associate with mRNA-binding proteins to form messenger ribonucleoprotein (mRNP) complexes. Recently, global proteomic and transcriptomic studies have provided comprehensive inventories of mRNP components. However, knowledge of the molecular features of distinct mRNP populations has remained elusive. We purifi ...[more]

PMID: 37399331

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