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Uncovering the positive role of strigolactone perception in barley drought response I

ABSTRACT: Drought is a destructive abiotic stress, with plants possessing diverse strategies to survive periods of limited water resources. Previous results have described connections between strigolactone (SL) and drought, however, here we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to investigate the SL-dependent mechanisms related to water deficit response. By combining transcriptome, proteome with phytohormone analyses and physiological data, we describe the drought-mediated differences between wild-type and hvd14 plants. Our findings indicate that the drought sensitivity of hvd14 is related to weaker induction of abscisic acid-responsive genes/proteins, lower jasmonic acid content, higher reactive oxygen species content, and lower wax biosynthic and deposition mechanisms then wild-type plants. In addition, we identify a series of transcription factors (TFs) that are exclusively drought-induced in wild-type barley. Critically, we resolve a comprehensive series interestions between the drought-induced barley transcriptome and proteome responses that allow us to understand the impacts of SL in mitigating water limiting conditions. These data provide a number of new angles for the development of drought-resistant barley.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Richard Uhrig

LAB HEAD: Richard Glen Uhrig

PROVIDER: PXD040828 | Pride | 2023-08-31

REPOSITORIES: Pride

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