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Determination of protein monoclonal-antibody epitopes by a combination of structural proteomics methods.

ABSTRACT: We use a combination of multiple structural proteomics techniques (differential photo-reactive surface modification, HDX, crosslinking and epitope excision/extraction) for the determination of the protein antigen epitopes for monoclonal antibodies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Hiv-1 M:b_mn

SUBMITTER: Evgeniy Petrotchenko

LAB HEAD: Christoph Borchers

PROVIDER: PXD040902 | Pride | 2023-10-24

REPOSITORIES: Pride

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Determination of Protein Monoclonal-Antibody Epitopes by a Combination of Structural Proteomics Methods.

Petrotchenko Evgeniy V EV Nascimento Elisabete M EM Witt Jody Melton JM Borchers Christoph H CH

Journal of proteome research 20230801 9

Structural proteomics techniques are useful for the determination of protein interaction interfaces. Each technique provides orthogonal structural information on the structure and the location of protein interaction sites. Here, we have characterized a monoclonal antibody epitope for a protein antigen by a combination of differential photoreactive surface modification (SM), cross-linking (CL), differential hydrogen-deuterium exchange (HDX), and epitope extraction/excision. We found that experime ...[more]

PMID: 37526474

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