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Mapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth

ABSTRACT: The aim of the project was to elucidate the inter-organellar interplay of plastids, mitochondria, and peroxisomes during storage reserve mobilization in the endosperm to supply the growing seedling with nutrients upon germination. Organelles from endosperm of etiolated castor bean seedlings were isolated and subjected to liquid chromatography-tandem mass spectrometry. The data were used to build a comprehensive metabolic model for plastids, mitochondria, and peroxisomes.

INSTRUMENT(S): Exactive Plus

ORGANISM(S): Ricinus Communis

TISSUE(S): Photosynthetic Cell

SUBMITTER: Anja Stefanski

LAB HEAD: Kai Stühler

PROVIDER: PXD040932 | Pride | 2024-01-09

REPOSITORIES: Pride

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Mapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth.

Wrobel Thomas J TJ Brilhaus Dominik D Stefanski Anja A Stühler Kai K Weber Andreas P M APM Linka Nicole N

Frontiers in plant science 20231006

In this work, we studied castor-oil plant <i>Ricinus communis</i> as a classical system for endosperm reserve breakdown. The seeds of castor beans consist of a centrally located embryo with the two thin cotyledons surrounded by the endosperm. The endosperm functions as major storage tissue and is packed with nutritional reserves, such as oil, proteins, and starch. Upon germination, mobilization of the storage reserves requires inter-organellar interplay of plastids, mitochondria, and peroxisomes ...[more]

PMID: 37868318

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