Dataset Information

Identification and functional characterization of NCoR1-interacting proteins associated with mitochondrial biogenesis

ABSTRACT: This study was designed to identify putative NCoR1-interacting proteins that might regulate the mitochondrial biogenesis process. To do that, MEF cells were transiently transfected with plasmid expressing either pcDNA-GFP-FLAG (control) or pCMX-NCoR1-FLAG and subjected to co-IP and identification of interacting proteins by liquid chromatography tandem mass spectrometry (LC-MS/MS) (LTQ Velos Orbitrap).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Adriana Franco Paes Leme

LAB HEAD: Leonardo Silveira

PROVIDER: PXD040945 | Pride | 2023-07-20

REPOSITORIES: Pride

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Publications

Interaction between poly(A)-binding protein PABPC4 and nuclear receptor corepressor NCoR1 modulates a metabolic stress response.

Oliveira A G AG Oliveira L D LD Cruz M V MV Guimarães D S P S F DSPSF Lima T I TI Santos-Fávero B C BC Luchessi A D AD Pauletti B A BA Leme A P AP Bajgelman M C MC Afonso J J Regitano L C A LCA Carvalho H F HF Carneiro E M EM Kobarg J J Perissi V V Auwerx J J Silveira L R LR

The Journal of biological chemistry 20230412 6

Mitochondria are organelles known primarily for generating ATP via the oxidative phosphorylation process. Environmental signals are sensed by whole organisms or cells and markedly affect this process, leading to alterations in gene transcription and, consequently, changes in mitochondrial function and biogenesis. The expression of mitochondrial genes is finely regulated by nuclear transcription factors, including nuclear receptors and their coregulators. Among the best-known coregulators is the ...[more]

PMID: 37059182

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Project description:Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the whole root and shoot of 7-d-old Arabidopsis thaliana seedlings. The cell type specific mRNA populations are those present in ribosome-mRNA complexes. This sub-population of mRNAs was obtained by first establishing a collection of Arabidopsis lines that express a FLAG-epitope tagged ribosomal protein L18 (RPL18) directed by promoters expressed in specific cell types and regions. Thirteen different promoter:FLAG-RPL18 lines were used. The targeted cell types and promoters included root atrichoblast (non-hair) epidermal cells (pGL2), root endodermis (pSCR), root stelar xylem and pericycle (pWOL, pSHR), root phloem companion cells (phloem CC) (pSUC2, pSultr2;2), root proliferating cells (pRPL11C), root cortex meristematic cells (pCO2), root cortex elongation/maturation cells (pPEP), shoot mesophyll (pRBCS), shoot epidermis (pCER5), shoot guard cells (pKAT1), shoot bundle sheath (pSultr2;2), shoot phloem CC (pSUC2) and shoot trichomes (pGL2). A CaMV 35S promoter:FLAG-RPL18 line was used to obtain the polysomal mRNA of multiple cell types. The immunopurification of ribosome-mRNA complexes of specific cell types/regions was accomplished by the method described in Zanetti et al. (Plant Physiology, 138, 624-635; 2005). Hybridization of the immunopurified mRNAs to the Affymetrix ATH1 DNA microarray platform and subsequent data analysis permitted the identification of transcripts that are enriched or depleted in specific cell types/regions of roots and shoots. The dataset includes samples from cell types/regions from seedlings grown under control conditions and cell types/regions of seedlings exposed to low oxygen stress (hypoxia) for 2 h. Experiment Overall Design: 79 samples, 2 conditions (2 h hypoxia stress, 2 h non-stress), 2 RNA pools (Total mRNA and polysomal mRNA), 2 organs, 13 promoter lines, 2-4 replicates

Dataset Information

Identification and functional characterization of NCoR1-interacting proteins associated with mitochondrial biogenesis

Publications

Interaction between poly(A)-binding protein PABPC4 and nuclear receptor corepressor NCoR1 modulates a metabolic stress response.

Similar Datasets

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