Identification of Snf2L-associated chromatin remodeling complex subunits in Plasmodium falciparum

ABSTRACT: ATP-dependent chromatin remodeling enzymes re-position and evict nucleosomes at specific genomic loci and therefore are essential regulators of all DNA dependent processes. They act in the context of large multiprotein complexes. The malaria-causing parasite Plasmodium falciparum (Pf) possesses a reduced set of chromatin remodeling enzymes, which are highly divergent, and no associated complex subunits are known. Within a detailed characterization of the ISWI-type remodeler PfSnf2L (PF3D7_1104200), potential interactors were identified. The protein was tagged endogenously in the parasite and co-immunoprecipitated. Subsequent LC-MS/MS analysis provided a list of specific protein interactors with a strong link to chromatin organization including nucleosome assembly factors, transcription factors as well as Plasmodium-specific uncharacterized proteins. The results suggest a functional role of PfSnf2L in nucleosome assembly and transcription regulation and point towards novel Plasmodium-specific chromatin remodeling complexes.

INSTRUMENT(S):

ORGANISM(S): Plasmodium Falciparum

SUBMITTER: Ignasi Forne  

LAB HEAD: Prof Dr Gernot Längst

PROVIDER: PXD041155 | Pride | 2024-10-17

REPOSITORIES: Pride