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Sirtuin 2 Promotes HCMV Replication by Regulating Cell Cycle Progression

ABSTRACT: The host cell cycle is a major target for manipulation by viral pathogens, which often replicate in certain cell cycle stages. The prevalent pathogen human cytomegalovirus (HCMV), for instance, only replicates during G1 and therefore employs diverse mechanisms for regulating cell cycle progression. Here, we demonstrate that the human enzyme sirtuin 2 (SIRT2) is required for HCMV-mediated cell cycle dysregulation. By combining virology assays with mass pectrometry-based proteome, acetylome, and interactome analyses, we have defined a pro-virus function for SIRT2 in regulating the G1 to S phase transition.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast

SUBMITTER: Cora Betsinger

LAB HEAD: Ileana M. Cristea

PROVIDER: PXD041171 | Pride | 2023-10-13

REPOSITORIES: Pride

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Sirtuin 2 promotes human cytomegalovirus replication by regulating cell cycle progression.

Betsinger Cora N CN Justice Joshua L JL Tyl Matthew D MD Edgar Julia E JE Budayeva Hanna G HG Abu Yaa F YF Cristea Ileana M IM

mSystems 20231102 6

<h4>Importance</h4>This study expands the growing understanding that protein acetylation is a highly regulated molecular toggle of protein function in both host anti-viral defense and viral replication. We describe a pro-viral role for the human enzyme SIRT2, showing that its deacetylase activity supports HCMV replication. By integrating quantitative proteomics, flow cytometry cell cycle assays, microscopy, and functional virology assays, we investigate the temporality of SIRT2 functions and sub ...[more]

PMID: 37916830

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