Dataset Information

Therapeutic CD14 heart proteome

ABSTRACT: Therapeutic remodelling and repair of murine heart induced by CD14 antibody treatment. Global (apex) heart and macrophage-derived (FACS) proteome remodelling of CD14 antibody-based therapeutic.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart, Macrophage

DISEASE(S): Myocardial Ischemia

SUBMITTER: david greening

LAB HEAD: David Greening

PROVIDER: PXD041192 | Pride | 2026-01-19

REPOSITORIES: Pride

ACCESS DATA

Publications

CD14 Blockade Modulates Macrophage-Mediated Immunological Injury in a Translational Model of Reperfused ST-Segment Elevation Myocardial Infarction.

D'Elia Nee Brown Aascha A AA Kiriazis Helen H Bloom Jason J Noonan Jonathan J Hsu Ian I Farrugia Gabriella E GE Fang Haoyun H Jansen Stephanie S Carvajal Natalia N Krstevski Crisdion C Shihata Waled A WA Tham Yow Keat YK Vais Angela A Cohen Camilla C Parslow Adam C AC Johnson Chad C Thomas Anita C AC Dona Malathi S I MSI Grigolon Kyah K Loh Scott J Y SJY Krippner Guy G Wright David K DK Wang Bing H BH Abbate Antonio A Amrute Junedh J Lavine Kory K Appleby Mark W MW Crowe David D Redlich Garry G Ziegelaar Brian W BW McMullen Julie R JR Greening David W DW Pinto Alexander R AR Kaye David M DM Donner Daniel G DG

JACC. Basic to translational science 20251023 11

These preclinical trials provide the first evidence of cluster of differentiation 14 (CD14) blockade with a murine analogue of atibuclimab, a CD14-neutralizing antibody, preventing secondary immunological exacerbation of cardiac injury in a translational mouse model of reperfused ST-segment elevation myocardial infarction (STEMI), assessed using multiple clinical modalities. Multiomic studies suggest CD14 blockade downregulated macrophage-specific proinflammatory and tissue-wide remodeling proce ...[more]

PMID: 41134248

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Project description:The abstract of the manuscript titled "Signalling pathways involved in adult heart formation revealed by gene expression profiling in Drosophila" is given below: "Drosophila provides a powerful system for defining the complex genetic programs that drive organogenesis. Under control of the steroid hormone ecdysone, the adult heart in Drosophila forms during metamorphosis by a remodelling of the larval cardiac organ. Here, we evaluated the extent to which transcriptional signatures revealed by genomic approaches can provide new insights into the molecular pathways that underlie heart organogenesis. Whole-genome expression profiling at 8 successive time-points covering adult heart formation revealed a highly dynamic temporal map of gene expression through 13 transcript clusters with distinct expression kinetics. A functional atlas of the transcriptome profile strikingly points to the genomic transcriptional response of the ecdysone cascade, and a sharp regulation of key components belonging to a few evolutionary conserved signalling pathways. A reverse genetic analysis provided evidence that these specific signalling pathways are involved in discrete steps of adult heart formation. In particular, the Wnt signalling pathway is shown to participate in inflow tract and cardiomyocyte differentiation, while activation of the PDGF-VEGF pathway is required for cardiac valve formation. Thus, a detailed temporal map of gene expression can reveal signalling pathways responsible for specific developmental programs and provides here substantial grasp into heart formation." Keywords: Time-course analysis Total RNAs were prepared from dissected cardiac tubes of Drosophila staged pupae at 8 successive time-points 21, 24, 27, 30, 33, 36, 42 and 48 hours after puparium formation covering adult heart organogenesis. For each time-point sample, 5 cardiac tubes were hand dissected from staged individuals. Four independent biological replicates were analyzed to confer a high reproducibility and statistical significance of the expression data. PolyA+ RNAs were linearly amplified (32 amplifications), labelled (32 with Cy3 and 32 with Cy5), and used for hybridization on Drosophila high-density oligonucleotide (INDAC) dual-channel microarrays (32 arrays) according to a loop-design dedicated to time-course experiments. Given the same number of arrays (8), a simple loop design is more efficient than a reference design: direct comparisons, smallest variance for log ratios, balancing varieties with dye-swapping (including technical replicates) and no reference sample needed (which has no intrinsic interest in our study). A graphical representation of the loop design used on this study is showed in the associated manuscript.

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Dataset Information

Therapeutic CD14 heart proteome

Dataset's files

Publications

CD14 Blockade Modulates Macrophage-Mediated Immunological Injury in a Translational Model of Reperfused ST-Segment Elevation Myocardial Infarction.

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