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Alternative mRNA splicing controls functions of the human H3K27 demethylase UTX/KDM6A

ABSTRACT: The UTX/KDM6A gene encodes the UTX histone H3K27 demethylase, which plays an important role in mammalian development and is frequently mutated in cancers and particularly, in urothelial cancers. Using BioID technique, we explored the interactome of different UTX isoforms.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Marc Timmers

LAB HEAD: Marc Timmers

PROVIDER: PXD041254 | Pride | 2024-01-28

REPOSITORIES: Pride

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Alternative mRNA Splicing Controls the Functions of the Histone H3K27 Demethylase UTX/KDM6A.

Fotouhi Omid O Nizamuddin Sheikh S Falk Stephanie S Schilling Oliver O Knüchel-Clarke Ruth R Biniossek Martin L ML Timmers H T Marc HTM

Cancers 20230608 12

The UTX/KDM6A histone H3K27 demethylase plays an important role in development and is frequently mutated in cancers such as urothelial cancer. Despite many studies on UTX proteins, variations in mRNA splicing have been overlooked. Using Nanopore sequencing, we present a comprehensive analysis of UTX/KDM6A splicing events in human cell lines and in tissue samples from bladder cancer cases and normal epithelia. We found that the central region of UTX mRNAs encoded by exons 12 to 17 undergoes exten ...[more]

PMID: 37370727

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