In planta deglycosylation improves the SARS-CoV-2 neutralization activity of recombinant ACE2-Fc
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ABSTRACT: The ectodomain of human ACE2-Fc was transiently expressed in leaves of Nicotiana benthamiana plants and protein A and SEC purified. SEC-purified ACE2-Fc was sequentially digested with chymotrypsin and trypsin and the glycopeptides were subjected to LC-ESI-MS analysis using the LC-OTMS, Orbitrap Exploris 480 from Thermo Scientific.
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Nicotiana Benthamiana
TISSUE(S): Epidermal Cell, Leaf
SUBMITTER: Richard Strasser
LAB HEAD: Richard Strasser
PROVIDER: PXD041320 | Pride | 2023-04-18
REPOSITORIES: Pride
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