In planta deglycosylation improves the SARS-CoV-2 neutralization activity of recombinant ACE2-Fc

ABSTRACT: The ectodomain of human ACE2-Fc was transiently expressed in leaves of Nicotiana benthamiana plants and protein A and SEC purified. SEC-purified ACE2-Fc was sequentially digested with chymotrypsin and trypsin and the glycopeptides were subjected to LC-ESI-MS analysis using the LC-OTMS, Orbitrap Exploris 480 from Thermo Scientific.

INSTRUMENT(S):

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Epidermal Cell, Leaf

SUBMITTER: Richard Strasser  

LAB HEAD: Richard Strasser

PROVIDER: PXD041320 | Pride | 2023-04-18

REPOSITORIES: Pride