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In planta deglycosylation improves the SARS-CoV-2 neutralization activity of recombinant ACE2-Fc

ABSTRACT: The ectodomain of human ACE2-Fc was transiently expressed in leaves of Nicotiana benthamiana plants and protein A and SEC purified. SEC-purified ACE2-Fc was sequentially digested with chymotrypsin and trypsin and the glycopeptides were subjected to LC-ESI-MS analysis using the LC-OTMS, Orbitrap Exploris 480 from Thermo Scientific.

INSTRUMENT(S):

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Epidermal Cell, Leaf

SUBMITTER: Richard Strasser

LAB HEAD: Richard Strasser

PROVIDER: PXD041320 | Pride | 2023-04-18

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ACE2Fc1379.dat	Other
	RST_1379.raw	Raw
	RST_1385.raw	Raw
	RST_1515.raw	Raw
	STEndoH1515.dat	Other

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