Proteomics

Dataset Information

0

Capillary Zone Electrophoresis-High Field Asymmetric Ion Mobility Spectrometry-Tandem Mass Spectrometry for Top-down Characterization of Histone Proteoforms

ABSTRACT: Characterization of histone proteoforms with various post-translational modifications (PTMs) is critical for a better understanding of functions of histone proteoforms in epigenetic control of gene expression. Mass spectrometry (MS)-based top-down proteomics (TDP) is a valuable approach for delineating histone proteoforms because it can provide us with a bird's-eye view of histone proteoforms carrying diverse combinations of PTMs. Here, we present the first example of coupling capillary zone electrophoresis (CZE), ion mobility spectrometry (IMS), and MS for online multi-dimensional separations of histone proteoforms. Our CZE-high-field asymmetric waveform IMS (FAIMS)-MS/MS platform identified 366 histone proteoforms from a commercial calf histone sample using a low microgram amount of histone sample as the starting material. CZE-FAIMS-MS/MS improved the number of histone proteoform identifications by about 3 folds compared to CZE-MS/MS alone (without FAIMS). The results indicate that CZE-FAIMS-MS/MS could be a useful tool for comprehensive characterization of histone proteoforms with high sensitivity.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Liangliang Sun  

LAB HEAD: Liangliang Sun

PROVIDER: PXD041357 | Pride | 2023-12-07

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Capillary zone electrophoresis-high field asymmetric ion mobility spectrometry-tandem mass spectrometry for top-down characterization of histone proteoforms.

Wang Qianyi Q   Fang Fei F   Wang Qianjie Q   Sun Liangliang L  

Proteomics 20231114 3-4

Characterization of histone proteoforms with various post-translational modifications (PTMs) is critical for a better understanding of functions of histone proteoforms in epigenetic control of gene expression. Mass spectrometry (MS)-based top-down proteomics (TDP) is a valuable approach for delineating histone proteoforms because it can provide us with a bird's-eye view of histone proteoforms carrying diverse combinations of PTMs. Here, we present the first example of coupling capillary zone ele  ...[more]

Similar Datasets

Proteomics Capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins
2023-12-07 | PXD042298 | Pride
Proteomics Towards a universal sample preparation method for denaturing top-down proteomics of complex proteomes
2020-05-22 | PXD018248 | Pride
Proteomics Laser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain
2023-03-11 | PXD027741 | Pride
Proteomics Capillary zone electrophoresis-tandem mass spectrometry with activated ion electron transfer dissociation for large-scale top-down proteomics
2019-06-11 | PXD012247 | Pride
Proteomics Qualitative and quantitative top-down proteomics of human colorectal cancer cell lines identified 23000 proteoforms and revealed drastic proteoform-level differences between metastatic and non-metastatic cancer cells
2023-01-05 | PXD029703 | Pride
Proteomics Predicting electrophoretic mobility of proteoforms for large-scale top-down proteomics
2020-02-17 | PXD017265 | Pride
Proteomics Single-shot top-down proteomics with capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for identification of nearly 600 Escherichia coli proteoforms
2017-10-30 | PXD007273 | Pride
Proteomics Capillary zone electrophoresis-tandem mass spectrometry as an alternative to liquid chromatography-tandem mass spectrometry for top-down proteomics of histones with the identification of over 1000 histone proteoforms
2022-04-04 | PXD021889 | Pride
Proteomics Pilot evaluation of long-term reproducibility of capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of a complex proteome sample
2024-03-20 | PXD046651 | Pride
Proteomics Characterization of human sperm protamine proteoforms through a combination of top-down and bottom-up mass spectrometry approaches
2019-11-11 | PXD014618 | Pride