Name: APEX proximity labeling of murine NDUFS4 interacting proteins in podocytes
Keywords: Apex proximity labeling of murine ndufs4 interacting proteins in podocytes

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APEX proximity labeling of murine NDUFS4 interacting proteins in podocytes

ABSTRACT: In this project we used an unbiased approach to identify the proteins that interact with NDUFS4 in the mouse podocytes using the APEX2 proximity labeling system. An immortalized murine podocyte was permanently transduced with a doxycycline (DOX) inducible NDUFS4-APEX2 chimeric construct. The cells were treated with DOX for 72 h to induce NDUFS4-APEX2 expression, followed by H2O2 activation of APEX2 mediated biotinylation of neighboring proteins. Biotinylated proteins were pull down by streptavidin beads and analyzed by LC-MS/MS. Cells without DOX induction or with DOX but without H2O2 activation were used as controls

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Antrix Jain

LAB HEAD: Farhad R. Danesh

PROVIDER: PXD041378 | Pride | 2024-02-08

REPOSITORIES: Pride

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