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Proteomic analysis of synovial fibroblasts from patients with Juvenile idiopathic arthritis

ABSTRACT: The aim was to characterize the proteomic alterations in synovial fibroblasts from patients with juvenile idiopathic arthritis, with or without prior priming with inflamed synovial fluid. Synovial fibroblasts were isolated from the synovial fluid of patients with oligoarticular juvenile idiopathic arthritis via passaging. For the experiment, they were primed or not with a pool of 20% of pooled synovial fluid from patients for 48hrs.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Synovial Fibroblast, Fibroblast

DISEASE(S): Juvenile Rheumatoid Arthritis

SUBMITTER: Charlotte Welinder

LAB HEAD: Tobias Schmidt

PROVIDER: PXD041459 | Pride | 2026-04-13

REPOSITORIES: Pride

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			Action	DRS
	TS10_CW_X2301_144.mzML	Mzml
	TS10_CW_X2301_144.raw	Raw
	TS11_CW_X2301_146.mzML	Mzml
	TS11_CW_X2301_146.raw	Raw
	TS12_CW_X2301_148.mzML	Mzml

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Publications

Synovial fluid potentiates local fibroblasts to drive monocyte activation in juvenile idiopathic arthritis.

Schmidt Tobias T Mossberg Anki A Król Petra P Kapetanovic Meliha C MC Einarsson Jon T JT Croft Adam P AP Bengtsson Anders A AA Kahn Fredrik F Kahn Robin R

Arthritis research & therapy 20260228 1

INTRODUCTION: Synovial fibroblasts (S-Fib) are recognized as key drivers of joint inflammation in adult arthritis. Moreover, data are limited on the processes driving monocyte activation in oligoarticular juvenile idiopathic arthritis (oJIA). Therefore, we aimed to explore if S-Fib from patients with oJIA induce monocyte activation. METHODS: S-Fib were isolated from the synovial fluid (SF) of n = 13 patients with oligoarticular juvenile idiopathic arthritis (oJIA) and n = 4 patients with rheumat ...[more]

PMID: 41761349

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Project description:Analysis of neutrophil proteomic alterations induced by migration towards inflamed joints in juvenile idiopathic arthritis (JIA). In this experiment neutrophil proteomes were investigated after migration towards JIA synovial fluid in an in vitro model of a synovial membrane, compared to neutrophils incubated in synovial fluid without migration. The migration model consisted of transwell inserts with human knee synoviocytes on the undersides and HMEC endothelial cells on the insides, placed in wells containing medium with 10 % JIA synovial fluid.

2022-02-15 | PXD029129 | Pride

Transcriptome profiling to identify a human effector regulatory T cell core signature

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Conventional DC1 (cDC1) are Strongly Enriched, Quiescent and Relatively Tolerogenic in Local Inflammatory Arthritis

Project description:We sequenced (bulk RNA-seq) conventional (c)DC1, cDC2 and monocytes from a local inflammatory site, namely synovial fluid (SF) from patients suffering from a chronic inflammatory condition, Juvenile Idiopathic Arthritis (JIA). To be able to compare to steady state, we also sequenced cDC2 and monocyte subsets from peripheral blood of healthy controls.

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Epigenetic profiling to identify a human effector regulatory T cell core signature [H3K27ac]

Project description:We investigated the active enhancer landscape of human Treg in an autoimmune-associated inflammatory environment, i.e. synovial fluid (SF) from inflamed joints of Juvenile Idiopathic Arthritis (JIA) to improve our understanding of human effector Treg programming.

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Epigenetic profiling to identify a human effector regulatory T cell core signature [H3K4me1]

Project description:We investigated the gene expression profile and active enhancer landscape of human Treg in an autoimmune-associated inflammatory environment, i.e. synovial fluid (SF) from inflamed joints of Juvenile Idiopathic Arthritis (JIA) to improve our understanding of human effector Treg programming.

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Global profiling of microRNAs in peripheral blood and synovial fluid mononuclear cells of patients with Enthesitis Related Arthritis

Project description:Enthesitis related arthritis (ERA) is the most common category of juvenile idiopathic arthritis in Asia. MicroRNA dysregulation has been shown in chronic arthritides however there is no data in ERA. MicroRNA dysregulation in ERA may help understand the pathogenesis of disease. Thus we determined the difference in global miRNA expression in peripheral blood mononuclear cells (PBMCs) of patients with ERA (n=8) as compared to healthy controls (HC) (n=8) and further to understand the changes at the site of inflammation, the PBMCs profile was compared with the synovial fluid mononuclear cells (SFMCs) (n=8) of patients with ERA.

2017-10-30 | GSE79481 | GEO

Proteome characterization of synovial fluid polarized healthy monocytes

Project description:This project involves proteome characterization of healthy monocytes polarized (activated) with synovial fluid (SF) versus serum from patients with juvenile idiopathic arthritis (JIA). The objective is to investigate the influence of SF on monocytes at the proteome level, using serum polarized monocytes as a control. Monocytes were isolated from peripheral blood mononuclear cells of healthy donors following negative selection and were subsequently polarized overnight with 20% of a pool of either serum or SF. They were subsequently detached and used for proteome analysis.

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Distinct cellular and spatial niches within the inflamed synovium of childhood arthritis [bulk RNA-Seq]

Project description:recision use of targeted therapies is urgently needed to improve long-term clinical outcomes for children affected by inflammatory arthritis, known as Juvenile Idiopathic Arthritis. Progress has been obstructed by a lack of understanding of the cellular basis of joint inflammation in children, given the difficulties in obtaining and studying synovial tissue itself. To this end, we combine single-cell RNA-sequencing, multiplexed immunofluorescence imaging and spatial transcriptomics to define the cellular and transcriptomic landscape of the synovium in children with Juvenile Idiopathic Arthritis. We identify spatial niches of resident and infiltrating cell populations that correlate with the degree of inflammation, and gene programs associated with arthritis severity. Combined with analyses of synovial fluid and peripheral blood from the same children, we distinguish differences in cellular composition, signalling pathways and transcriptional programs across anatomical compartments. Whilst we identify several pathogenic populations shared with adult-onset arthritis, our analyses highlight increased vascularity of the inflamed developing joint and TGFb-driven stromal subsets that upregulate expression of disease risk-associated genes. Overall, these findings illustrate the need for treatment algorithms informed by a tissue-based classification of arthritis.

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Distinct cellular and spatial niches within the inflamed synovium of childhood arthritis [scRNA-Seq]

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Effect of T cell supernatants or cytokines on synovial fibroblasts

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