Dataset Information

TFT3 interacts with MAPKKK by coimmunoprecipitation

ABSTRACT: Tomato MAPKKK interacts with the N. benthamiana homologue of TFT3. MAPKKK-HA was transiently expressed in N. benthamiana leaves and immunoprecipitated using -HA agarose beads. 12 unique peptides of N. benthamiana TFT3 homologue were identified by liquid chromatography–mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Alex Jones

LAB HEAD: Alex Jones

PROVIDER: PXD041469 | Pride | 2023-05-10

REPOSITORIES: Pride

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Publications

Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition.

Sheikh Arsheed H AH Zacharia Iosif I Pardal Alonso J AJ Dominguez-Ferreras Ana A Sueldo Daniela J DJ Kim Jung-Gun JG Balmuth Alexi A Gutierrez Jose R JR Conlan Brendon F BF Ullah Najeeb N Nippe Olivia M OM Girija Anil M AM Wu Chih-Hang CH Sessa Guido G Jones Alexandra M E AME Grant Murray R MR Gifford Miriam L ML Mudgett Mary Beth MB Rathjen John P JP Ntoukakis Vardis V

Nature communications 20230504 1

In both plants and animals, nucleotide-binding leucine-rich repeat (NLR) immune receptors play critical roles in pathogen recognition and activation of innate immunity. In plants, NLRs recognise pathogen-derived effector proteins and initiate effector-triggered immunity (ETI). However, the molecular mechanisms that link NLR-mediated effector recognition and downstream signalling are not fully understood. By exploiting the well-characterised tomato Prf/Pto NLR resistance complex, we identified th ...[more]

PMID: 37142566

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Project description:RNA from the aerial parts of 15-day-old eceriferum cer6-2 mutant and control plants were used in microarray experiments to screen for differentially expressed genes. Among those which were down regulated in the mutant, several were known to be involved in various plant defense mechanisms. The most remarkable difference was measured for the transcripts encoding the pathogenesis-related protein1 (PR-1), which is a marker of the systemic acquired resistance (SAR) pathway, with a 100-fold decrease in the cer6-2 mutant. Further Q-PCR experiments showed that the PR-1 transcript levels were low in two other eceriferum mutants, representing 1/200th and 1/25th of the normal values in the aerial part of 15-day-old cer2 and cer1-1 mutants, respectively. Interestingly, PR-1 mRNA levels were closed to the normal values in the cer3-1 and cer6-2R mutant, the latter being a plant where the synthesis of the very-long chain fatty acids, which is deficient in the cer6-2 plants, is partially restored. Altogether, these results strongly suggested that the amount of PR1-mRNA was not directly correlated to the amount of epicuticular wax on the leaves, but was rather correlated to the presence or absence of some particular lipid-constituents in the epicuticular wax layer. Related publication:; Garbay B, Tautu MT, Costaglioli P. Low level of pathogenesis-related protein 1 mRNA expression in 15-day-old Arabidopsis cer6-2 and cer2 eceriferum mutants. Plant Science 2007; Volume 172, Issue 2, Pages 299-305 Experiment Overall Design: Total RNA was purified from the aerial parts of 2-week-old plants (20 plants were pooled for each genotype). Arabidopsis plants were grown under long-day conditions (16 h light:8 h dark) at 22Â°C and 80% relative humidity. The control plants and the cer mutant were cultivated in the same phytotron, and the Petri dishes were placed randomly on the same shelf. This was done to avoid any difference in plant growth conditions between the mutants and the control plants. The microarrays used were Agilent Arabidopsis2. GSM87735 represents the direct experiment (cer6-2 Cy3, Ler-0 Cy5), and GSM88340 represents the dye-swap (Ler-0 Cy3, cer6-2 Cy5)

Dataset Information

TFT3 interacts with MAPKKK by coimmunoprecipitation

Publications

Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition.

Similar Datasets

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