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Identification of proteins bound with RPA194 at rDNA loci post radiation in breast cancer cells

ABSTRACT: Following radiation damage, cells resume ribosome biogenesis for survival. We combined chromatin immunoprecipitation (ChIP) with mass spectrometry to identify key binding partners of RPA194 (the major subunit of RNA polI) at the rDNA loci in response to irradiation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Brandon Metge

LAB HEAD: Lalita Samant

PROVIDER: PXD041472 | Pride | 2023-07-20

REPOSITORIES: Pride

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Ribosome biosynthesis and Hedgehog activity are cooperative actionable signaling mechanisms in breast cancer following radiotherapy.

Metge Brandon J BJ Alsheikh Heba A HA Chen Dongquan D Elhamamsy Amr R AR Hinshaw Dominique C DC Chen Bo-Ruei BR Sleckman Barry P BP Samant Rajeev S RS Shevde Lalita A LA

NPJ precision oncology 20230628 1

Hyperactivated ribosome biosynthesis is attributed to a need for elevated protein synthesis that accommodates cell growth and division, and is characterized by nucleomorphometric alterations and increased nucleolar counts. Ribosome biogenesis is challenged when DNA-damaging treatments such as radiotherapy are utilized. Tumor cells that survive radiotherapy form the basis of recurrence, tumor progression, and metastasis. In order to survive and become metabolically revitalized, tumor cells need t ...[more]

PMID: 37380890

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