Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
TISSUE(S): Cell Culture
SUBMITTER: Eric Bonneil
LAB HEAD: Pierre Thibault
PROVIDER: PXD041524 | Pride | 2023-08-10
REPOSITORIES: Pride
Homsi Charles C Rajan Roshan Elizabeth RE Minati Robin R St-Hilaire Edlie E Bonneil Eric E Dufresne Simon F SF Wurtele Hugo H Verreault Alain A Thibault Pierre P
Journal of proteome research 20230718 8
Current protocols used to extract and purify histones are notoriously tedious, especially when using yeast cells. Here, we describe the use of a simple filter-aided sample preparation approach enabling histone extraction from yeast and mammalian cells using acidified ethanol, which not only improves extraction but also inactivates histone-modifying enzymes. We show that our improved method prevents N-terminal clipping of H3, an artifact frequently observed in yeast cells using standard histone e ...[more]