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Protein expression profiling in eggs and embryos from control and Eif4e1b knockout female mice.

ABSTRACT: We have identified eukaryotic translation initiation factor 4E family member 1B (Eif4e1b) as a regulator of maternal RNA translation, whose maternal deletion leads to 2-cell arrest phenotype in mouse embryos. eIF4E1b protein binds mRNA selectively in oocytes and controls their translation in early embryos. We aim to profile the global changes in protein expression in metaphase II (MII) eggs and zygotes collected from control or Eif4e1b knockout female mice. An encapsulated proteomic-sample processing protocol is used to perform this low-input mass spectrometry to quantify proteomic changes (Kulak et al. 2014/PMID 24497582).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo, Oocyte

SUBMITTER: David Anderson

LAB HEAD: Jurrien Dean

PROVIDER: PXD041583 | Pride | 2023-06-12

REPOSITORIES: Pride

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Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation.

Yang Guanghui G Xin Qiliang Q Feng Iris I Wu Di D Dean Jurrien J

Genes & development 20230531 9-10

Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remain unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent repro ...[more]

PMID: 37257918

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