OGA-mediated protein glycosylation regulates pluripotency states in human embryonic stem cells

ABSTRACT: In our previous study, we uncovered that OGA significantly elevated in naïve hESC. Therefore, we sought to investigate the effects of O-GlcNAc on hESC and the interconvert of both pluripotent states. Depletion of OGA, which results in global O-GlcNAcylation increased, would impair naïve hESC pluripotency but can promote naïve to primed hESC transition. In addition, we obtained the profiles of O-GlcNAcylated proteins in H9 primed hESC and H9-PXGL hESC via MS based-quantitative proteomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Zhiya Fan  

LAB HEAD: Qin Weijie

PROVIDER: PXD041605 | Pride | 2024-08-10

REPOSITORIES: Pride