Dataset Information

Identification of co-factors of eIF4E1b protein in mouse ovary.

ABSTRACT: We have identified eukaryotic translation initiation factor 4E family member 1B (Eif4e1b) as a regulator of maternal RNA translation, whose maternal deletion leads to 2-cell arrest phenotype in mouse embryos. eIF4E1b protein binds mRNA selectively in oocytes and controls their translation in early embryos. eIF4E1b expresses specifically in mouse ovary and we have generated a knock-in mouse line in which HA tag is conjugated to the C terminus of eIF4E1b. We aim to identify eIF4E1b co-factors using mass spectrometry after anit-HA immunoprecipitation (IP) using mouse ovaries.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Ovary

SUBMITTER: David Anderson

LAB HEAD: Jurrien Dean

PROVIDER: PXD041622 | Pride | 2023-06-12

REPOSITORIES: Pride

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Publications

Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation.

Yang Guanghui G Xin Qiliang Q Feng Iris I Wu Di D Dean Jurrien J

Genes & development 20230531 9-10

Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remain unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent repro ...[more]

PMID: 37257918

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Project description:A long-standing view of development is that transcription is silenced in the oocyte until early divisions in the embryo. The point at which major transcription is reactivated varies in different organisms, but is usually after the 2-cell stage. However, this model may not be universal. We used RNA-seq and exploited the protracted development of the parasitic nematode Ascaris suum, to provide a comprehensive time course of mRNA expression, degradation, and translation during early development. Surprisingly, we find that ~4,000 genes are transcribed prior to pronuclear fusion and in the 1-4 cell embryos. Intriguingly, we do not detect maternal contribution of many orthologs of maternal C. elegans mRNAs, but instead find these are newly transcribed in the A. suum zygote prior to pronuclear fusion. Ribosome profiling demonstrates that, in general, early embryonic mRNAs are not stored for subsequent translation, but are directly translated following their synthesis. The role of maternally contributed and zygotically transcribed genes differs between the nematodes A. suum and C. elegans despite the fact that the two nematodes appear to exhibit highly similar morphological patterns during early development. Our study indicates that major transcription can occur immediately after fertilization and prior to pronuclear fusion in metazoa, suggesting that newly transcribed genes appear to drive A. suum early development. Furthermore, the mechanisms used for controlling the timing of the expression of key conserved genes has been altered between the two nematodes, illustrating significant plasticity in the regulatory networks that play important roles in developmental outcomes in nematodes. A total of 28 samples are used for this study. The RNAs for the library construction are derived from 0.5 M-bM-^@M-^S 1 ml of synchronized embryo stages corresponding to a minimum of 0.5 billion embryos derived from > 1,000 female worms. The RNAs for polysome analysis are from pooled fractions of polysome gradient. For each sample, the amount of RNA for all polysome fractions is equivalent to the total RNA from the sample. Thus, the data from polysome profiling and RNA transcriptomes are considered as biological replicates. The level of the RNAs between each neighboring stages during development are considered as the reference for each other. Different sequencing strategies were applied for multiple libraries.

Dataset Information

Identification of co-factors of eIF4E1b protein in mouse ovary.

Publications

Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation.

Similar Datasets

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